Ultrasound patterning technologies for studying vascular morphogenesis in 3D
Ultrasound patterning technologies for studying vascular morphogenesis in 3D
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DOI：
10.1242/jcs.188151
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发表时间：
2017-01-01
影响因子：
4
通讯作者：
Dalecki, Diane
中科院分区：
文献类型：
--
作者：
Comeau, Eric S.;Hocking, Denise C.;Dalecki, Diane
这份报告中的研究展示了基于超声的模式和成像技术在研究3D环境中血管形态发生的决定因素方面的多功能性。利用与超声驻波场(USWF)相关的力在3D胶原水凝胶中无创地和体积地形成内皮细胞。图案化水凝胶由位于USWF结节区域的平行内皮细胞带组成，间隔等于入射声场的一半波长。调整声学参数以改变内皮带的空间尺寸，并分析其对微血管形态形成的影响。高频超声成像技术用于成像和量化初始平面细胞条带的间距、宽度和密度。对所得微血管网络的分析表明，血管宽度、取向、密度和分支活动强烈地受平面带的初始3D组织的影响，因此可以由用于图案化的声学参数来控制。总而言之，USWF图案化和高频超声成像工具的集成使得能够制造具有定义的微血管大小和方向的血管构造，从而深入了解3D中的空间线索如何影响血管形态发生。
Investigations in this report demonstrate the versatility of ultrasound-based patterning and imaging technologies for studying determinants of vascular morphogenesis in 3D environments. Forces associated with ultrasound standing wave fields (USWFs) were employed to non-invasively and volumetrically pattern endothelial cells within 3D collagen hydrogels. Patterned hydrogels were composed of parallel bands of endothelial cells located at nodal regions of the USWF and spaced at intervals equal to one half wavelength of the incident sound field. Acoustic parameters were adjusted to vary the spatial dimensions of the endothelial bands, and effects on microvessel morphogenesis were analyzed. High-frequency ultrasound imaging techniques were used to image and quantify the spacing, width and density of initial planar cell bands. Analysis of resultant microvessel networks showed that vessel width, orientation, density and branching activity were strongly influenced by the initial 3D organization of planar bands and, hence, could be controlled by acoustic parameters used for patterning. In summary, integration of USWF-patterning and high-frequency ultrasound imaging tools enabled fabrication of vascular constructs with defined microvessel size and orientation, providing insight into how spatial cues in 3D influence vascular morphogenesis.