Psg18 is specifically expressed in follicle-associated epithelium
Psg18 is specifically expressed in follicle-associated epithelium
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DOI：
10.1247/csf.07014
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发表时间：
2007-01-01
影响因子：
1.5
通讯作者：
Ohno, Hiroshi
中科院分区：
文献类型：
--
作者：
Kawano, Kazuya;Ebisawa, Masashi;Ohno, Hiroshi
胎盘分泌的妊娠特异性糖蛋白(PSGs)通过诱导抗炎细胞因子IL-10来调节免疫系统，以确保胎儿移植物的存活。然而，除了母体免疫外，PSG是否还参与免疫反应的更一般方面，目前尚不清楚。在这里，我们报告Psg18在覆盖Peyer‘s Patches(PPS)的滤泡相关上皮(Fae)中高表达。免疫细胞参考数据库(RefDIC)的生物信息学分析以及RT-PCR数据表明，Psg18在成年小鼠的FAE中特异表达，而其他PSG家族成员在FAE中不表达或仅轻微表达。Psg18在无菌小鼠的FAE中有表达，接种细菌后略有上调。原位杂交分析表明，Psg18在整个FAE中广泛表达。此外，Psg18蛋白沉积在FAE下上皮下穹顶的细胞外基质上，抗原提呈细胞在那里聚集。这些结果表明，Psg18是一种FAE特异性标记蛋白，可促进粘膜相关淋巴组织中FAE与免疫细胞之间的相互作用。
Pregnancy-specific glycoproteins (Psgs) secreted by the placenta regulate the immune system to ensure the survival of the fetal allograft by inducing IL-10, an anti-inflammatory cytokine. However, it is unknown whether Psgs are involved in more general aspects of immune response other than maternal immunity. Here, we report that Psg18 is highly expressed in the follicle-associated epithelium (FAE) overlaying Peyer's patches (PPs). Bioinformatics analysis with Reference Database for Immune Cells (RefDIC) as well as RT-PCR data demonstrated that Psg18 is exclusively expressed in FAE in adult mice, in contrast to other Psg family members that are either not expressed or only slightly expressed in FAE. Psg18 expression was observed in FAE of germ-free-conditioned mice, and was slightly upregulated after bacterial inoculation. In situ hybridization analysis revealed that Psg18 is widely expressed throughout FAE. Furthermore, Psg18 protein is deposited on the extracellular matrix in the subepithelial dome beneath FAE, where antigen-presenting cells accumulate. These results suggest that Psg18 is an FAE-specific marker protein that could promote interplay between FAE and immune cells in mucosa-associated lymphoid tissues.