Extraordinary proliferation of microorganisms in aposymbiotic pea aphids, Acyrthosiphon pisum
Extraordinary proliferation of microorganisms in aposymbiotic pea aphids, Acyrthosiphon pisum
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DOI：
10.1016/s0022-2011(03)00020-x
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发表时间：
2003-03-01
影响因子：
3.4
通讯作者：
Kudo, T
中科院分区：
文献类型：
--
作者：
Nakabachi, A;Ishikawa, H;Kudo, T
被剥夺了细胞内共生细菌 Buchnera 的非共生豌豆蚜表现出生长迟缓并且没有繁殖力。高效液相色谱（HPLC）分析表明，这些非共生蚜虫在蚕豆植物上饲养时，积累了大量的组胺。为了评估除 Buchnera 之外的微生物异常增殖的可能性，我们使用实时定量 PCR 方法对蚜虫中的真细菌和真菌进行了计数，该方法针对编码小亚基 rRNA 的基因。结果表明，这些微生物在植物上饲养的非共生蚜虫中极其丰富。通过小亚基 rDNA 的系统发育分析进一步分析了无性共生蚜虫中的微生物群落。在真菌 18S rDNA 的 172 个非嵌合序列中，138 个 (80.2%) 属于子囊菌门。其中，21 种聚集在由昆虫病原真菌和同翅目昆虫的类酵母共生体组成的单系群中。三十一个 (18.0%)、两个 (1.2%) 和一个 (0.6%) 克隆分别聚集在担子菌门、接合菌门和卵菌门内。在真细菌 16S rDNA 的 167 个非嵌合序列中，84 个（50.3%）属于变形菌门的 γ 亚门，昆虫和多产组胺生产者的大多数初级内共生体都属于该门。 40 个 (24.0%)、25 个 (15.0%)、10 个 (6.0%) 和 5 个 (3.0%) 克隆分别聚集在 α-变形菌门、噬细胞-黄杆菌-拟杆菌 (CFB) 群、放线菌门和 β-变形菌门内。其中三个与已知的分类学划分没有系统发育关联。本研究中研究的序列均与 GenBank 中存储的序列完全一致。 (C) 2003 年爱思唯尔科学（美国）。版权所有。
Aposymbiotic pea aphids, which were deprived of their intracellular symbiotic bacterium, Buchnera, exhibit growth retardation and no fecundity. High performance liquid chromatographic (HPLC) analysis revealed that these aposymbiotic aphids, when reared on broad bean plants, accumulated a large amount of histamine. To assess the possibility of extraordinary proliferation of microorganisms other than Buchnera, we enumerated eubacteria and fungi in aphids using the real-time quantitative PCR method that targets genes encoding small-subunit rRNAs. The result showed that these microorganisms were extremely abundant in the aposymbiotic aphids reared on plants. Microbial communities in aposymbiotic aphids were further profiled by phylogenetic analysis of small-subunit rDNAs. Of 172 nonchimeric sequences of fungal 18S rDNAs, 138 (80.2%) belonged to the phylum Ascomycota. Among them, 21 clustered within a monophyletic group consisting of insect-pathogenic fungi and yeast-like symbionts of homopteran insects. Thirty-one (18.0%), two (1.2%.), and one (0.6%) clones were clustered within the Basidiomycota, Zygomycota, and Oomycota, respectively. Of 167 nonchimeric sequences of eubacterial 16S rDNAs, 84 (50.3%) belonged to the gamma-subdivision of Proteobacteria to which most primary endosymbionts of insects and prolific histamine producers belong. Forty (24.0%), 25 (15.0%), 10 (6.0%), and five (3.0%) clones were clustered within alpha-Proteobacteria, Cytophaga-Flavobacterium-Bacteroides (CFB) group, Actinobacteria, and beta-Proteobacteria, respectively. Three had no phylogenetic association with known taxonomic divisions. None of the sequences studied in this study coincided exactly with those deposited in GenBank. (C) 2003 Elsevier Science (USA). All rights reserved.