Characterization of chemotaxis and motility response towards fructose in Escherichia coli
Characterization of chemotaxis and motility response towards fructose in Escherichia coli
复制标题
大肠杆菌对果糖的趋化性和运动性反应的表征
DOI：
10.1016/j.bbrc.2020.04.087
复制
发表时间：
2020
影响因子：
3.1
通讯作者：
Liu Jian
中科院分区：
文献类型：
--
作者：
Liu Fanghai;Wang Fangbin;Liu Jian
大肠杆菌（Escherichiacoli，E. coli）等围毛鞭毛细菌在鞭毛马达的驱动下，通过对各种化学物质的偏向性随机游走进行趋化。果糖是一种典型的单糖，对大肠杆菌具有吸引作用，但大肠杆菌对果糖的趋化性和运动性反应却知之甚少。本文采用微流控技术和磁珠法研究了大肠杆菌对0 mM ~ 50 mM不同浓度果糖的趋化行为。我们观察到野生型细胞对果糖的刺激有反应，表明果糖是大肠杆菌的引诱剂，而趋化缺陷型细胞则不能感受到果糖的刺激。在不同浓度的果糖中，野生型细胞的运动性降低，这有助于细胞在表面附近的聚集，与果糖对趋化性缺陷的细胞的运动性没有影响的结果相反。在其他单糖对大肠杆菌的作用中也可能发现类似的现象。
Peritrichously flagellated bacteria such asEscherichia coli(E. coli) perform chemotaxis by a biased random walk toward various chemicals, which was driven by the bacterial flagellar motor. Fructose, a typical monosaccharide that can attractE. coli.However, little is known about the chemotaxis and motility response ofE. colitowards fructose. Here, we characterized the chemotaxis behavior ofE. colito different concentrations of fructose from 0 mM to 50 mM by using microfluidics and bead assay. We observed the wild-type cells responded to the stimulus of fructose, which suggested fructose is an attractant toE. coli, while the cells defective in chemotaxis could not sense the stimulus of fructose. The motility of wild-type cells was reduced in various concentrations of fructose, which helped the aggregation of cells near surfaces, in contrast with the result that the fructose showed no effect on the motility of the cells defective in chemotaxis. Similar phenomena are expected to be found in the effect of other monosaccharides toE. coli.