Calcineurin-mediated BAD Ser155 dephosphorylation in arnmonia-induced apoptosis of cultured rat hippocampal neurons
Calcineurin-mediated BAD Ser155 dephosphorylation in arnmonia-induced apoptosis of cultured rat hippocampal neurons
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DOI：
10.1016/j.neulet.2003.12.032
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发表时间：
2004-02-26
影响因子：
2.5
通讯作者：
Inagaki, C
中科院分区：
文献类型：
--
作者：
Yang, L;Omori, K;Inagaki, C
我们以前报道，氨诱导培养的大鼠海马神经元细胞内钙离子浓度中度增加和磷酸化BAD水平下降的凋亡。由于这表明钙调神经磷酸酶参与细胞凋亡，因此测试了钙调神经磷酸酶抑制剂1 μ M环孢菌素A和1 μ M FK 506对氨诱导的神经元细胞凋亡的影响。通过Hoechst 33258和抗神经丝抗体的双重染色评估，两种抑制剂都消除了神经元凋亡，以及氨诱导的磷酸化BAD Ser（155）水平的降低。因此，钙调神经磷酸酶似乎参与了氨诱导的细胞凋亡中BAD在Ser（155）位点的去磷酸化。(C)2003爱思唯尔爱尔兰有限公司保留所有权利。
We previously reported that ammonia induced apoptosis in cultured rat hippocampal neurons with moderate increases in the intracellular calcium concentration and decreases in phospho-BAD levels. Since this suggested the involvement of calcineurin in the apoptosis, the effects of calcineurin inhibitors, 1 muM cyclosporin A and 1 muM FK506, on the ammonia-induced neuronal apoptosis were tested. Both of the inhibitors abolished the neuronal apoptosis assessed by double staining with Hoechst 33258 and anti-neurofilament antibody, and the ammonia-induced decrease in phospho-BAD Ser(155) level. Thus, calcineurin appeared to be involved in the dephosphorylation of BAD at the sites including Ser(155) in ammonia-induced apoptosis. (C) 2003 Elsevier Ireland Ltd. All rights reserved.