Zebrafish: A tool for chemical screens.
Zebrafish: A tool for chemical screens.
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斑马鱼：化学筛选工具。
DOI：
10.1002/bdrc.20183
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Tsang,Michael
中科院分区：
文献类型：
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作者：
Tsang,Michael
斑马鱼已被证明是发育生物学的宝贵脊椎动物模型。最近的技术进步增加了一系列工具，将其应用扩展到药物发现领域。这包括产生转基因报告细胞系的方法，该转基因报告细胞系允许活胚胎中的荧光标记物的直接可视化。随着小分子处理胚胎的自动成像和分析的增加，这些创新将其应用扩展到高通量化学筛选。这篇综述将重点介绍其中一些推动斑马鱼作为药物发现工具的进展。出生缺陷研究（C部分）90：185-192，2010年。© 2010 Wiley利斯公司
The zebrafish has proven to be an invaluable vertebrate animal model for developmental biology. Recent technological advances have added an arsenal of tools to expand its use into the realm of drug discovery. This includes methodology to generate transgenic reporter lines that allow for the direct visualization of fluorescent markers in live embryos. With the addition of automated imaging and analysis of embryos treated with small molecules, these innovations have expanded its utility into high throughput chemical screens. This review will highlight some of these advances that have propelled zebrafish as a tool for drug discovery. Birth Defects Research (Part C) 90:185–192, 2010. © 2010 Wiley‐Liss, Inc.