Genetic architecture of mitochondrial editing in Arabidopsis thaliana
Genetic architecture of mitochondrial editing in Arabidopsis thaliana
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DOI：
10.1534/genetics.107.073585
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发表时间：
2008-03-01
期刊：
影响因子：
3.3
通讯作者：
Hanson, Maureen R.
中科院分区：
文献类型：
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作者：
Bentolila, Stephane;Elliott, Leah E.;Hanson, Maureen R.
我们分析了两个拟南芥种质，兰茨贝格直立（Ler）和哥伦比亚（Col）的线粒体编辑行为。以莲座叶RNA为模板，对33个线粒体基因的362个C → U编辑位点进行了分析。我们在A中检测到67个新的编辑事件。在悬浮培养物中的线粒体编辑的先前研究中没有观察到的拟南芥莲座叶。此外，在A.在莲座叶中没有观察到拟南芥悬浮培养物。在Col和Ler之间检测到40个编辑程度多态的编辑位点。沉默的编辑网站，这不会改变编码的氨基酸，被发现在一个大的过剩相比，nonsilent网站之间的编辑事件，不同的加入和组织类型之间。显性关系进行了评估的15个最多态性的网站，通过评估的编辑值的相互杂交。显性是更常见的nonsilent网站比在沉默的网站，而加性只在沉默的网站。8个位点检测到母体效应。利用重组近交系的QTL作图检测了13个编辑性状中11个的12个主效QTL，表明单个线粒体C靶标的编辑效率通常由一个主效因子控制。
We have analyzed the mitochondrial editing behavior of two Arabidopsis thaliana accessions, Landsberg erecta (Ler) and Columbia (Col). A survey of 362 C-to-U editing sites in 33 mitochondrial genes was conducted on RNA extracted from rosette leaves. We detected 67 new editing events in A. thaliana rosette leaves that had not been observed in a prior study of mitochondrial editing in suspension cultures. Furthermore, 37 of the 441 C-to-U editing events reported in A. thaliana suspension cultures were not observed in rosette leaves. Forty editing sites that are polymorphic in extent of editing were detected between Col and Ler. Silent editing sites, which do not change the encoded amino acid, were found in a large excess compared to nonsilent sites among the editing events that differed between accessions and between tissue types. Dominance relationships were assessed for 15 of the most polymorphic sites by evaluating the editing values of the reciprocal hybrids. Dominance is more common in nonsilent sites than in silent sites, while additivity was observed only in silent sites. A maternal effect was detected for 8 sites. QTL mapping with recombinant inbred lines detected 12 major QTL for 11 of the 13 editing traits analyzed, demonstrating that efficiency of editing of individual rnitochondrial C targets is generally governed by a major factor.