BINDING AND SUPPRESSION OF THE MYC TRANSCRIPTIONAL ACTIVATION DOMAIN BY P107
BINDING AND SUPPRESSION OF THE MYC TRANSCRIPTIONAL ACTIVATION DOMAIN BY P107
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DOI：
10.1126/science.8146655
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发表时间：
1994-04-08
期刊：
影响因子：
56.9
通讯作者：
DALLAFAVERA, R
中科院分区：
文献类型：
--
作者：
GU, W;BHATIA, K;DALLAFAVERA, R
Myc作为一个转录因子控制细胞增殖、分化和凋亡，需要一个氨基末端的反式激活结构域。用Myc融合蛋白筛选互补DNA表达文库以鉴定与该结构域相互作用的蛋白质，并分离编码Rb相关p107蛋白的克隆。p107蛋白被证明与Myc在体内，并抑制Myc反式激活结构域的活性。然而，突变形式的Myc从伯基特淋巴瘤细胞，其中包含在反式激活结构域的序列改变，耐p107介导的抑制。因此，破坏Myc和p107之间的调节相互作用可能是重要的肿瘤发生。
An amino-terminal transactivation domain is required for Myc to function as a transcription factor controlling cell proliferation, differentiation, and apoptosis. A complementary DNA expression library was screened with a Myc fusion protein to identify proteins interacting with this domain, and a clone encoding the Rb-related p107 protein was isolated. The p107 protein was shown to associate with Myc in vivo and to suppress the activity of the Myc transactivation domain. However, mutant forms of Myc from Burkitt lymphoma cells, which contain sequence alterations in the transactivation domain, were resistant to p107-mediated suppression. Thus, disruption of a regulatory interaction between Myc and p107 may be important in tumorigenesis.