1H NMR-based metabolomics approach for exploring urinary metabolome modifications after acute and chronic physical exercise
1H NMR-based metabolomics approach for exploring urinary metabolome modifications after acute and chronic physical exercise
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DOI：
10.1007/s00216-009-3289-4
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发表时间：
2010-02-01
影响因子：
4.3
通讯作者：
Dugue, B.
中科院分区：
文献类型：
--
作者：
Enea, C.;Seguin, F.;Dugue, B.
代谢组学是一种代谢物评估的综合方法，涉及测量生物样品的整体代谢特征。我们使用这种方法来研究急性和慢性体育锻炼引起的生化变化。 22 名使用相同口服避孕药的女性根据她们的体能训练背景被分为未训练组 (n=10) 或训练过组 (n=12)。受试者以随机顺序进行两项运动：长时间运动测试（超过点 O-2 max 的 (V) 的 75%，直至精疲力竭）和短期强化运动测试（短期强化运动无氧测试）。每次测试前和测试后 30 分钟收集尿液样本。通过 H-1 NMR 光谱分析样品，并利用多元统计技术处理数据。仅在短期剧烈运动测试之前和之后 30 分钟收集的样本的尿液特征中观察到了区别特征。造成这种变化的代谢物是肌酐、乳酸、丙酮酸、丙氨酸、β-羟基丁酸、乙酸盐和次黄嘌呤。在两组中，完成短期强化运动测试后，乳酸、丙酮酸、丙氨酸、β-羟基丁酸和次黄嘌呤的排泄量增加相似（p
Metabolomics is a comprehensive method for metabolite assessment that involves measuring the overall metabolic signature of biological samples. We used this approach to investigate biochemical changes due to acute and chronic physical exercise. Twenty-two women using identical oral contraceptives were segregated into an untrained (n=10) or trained (n=12) group depending on their physical training background. The subjects performed two exercises in a randomized order: a prolonged exercise test (75% of their (V) over dot O-2 max until exhaustion) and a short-term, intensive exercise test (short-term, intensive exercise anaerobic test). Urine specimens were collected before and 30 min after each test. The samples were analyzed by H-1 NMR spectroscopy, and multivariate statistical techniques were utilized to process the data. Distinguishing characteristics were observed only in the urine profiles of specimens collected before vs. 30 min after the short-term, intensive exercise test. The metabolites responsible for such changes were creatinine, lactate, pyruvate, alanine, beta-hydroxybutyrate, acetate, and hypoxanthine. In both groups, the excretion of lactate, pyruvate, alanine, beta-hydroxybutyrate, and hypoxanthine increased similarly after the completion of the short-term, intensive exercise test (p