Transcriptional regulation of the AMP-activated protein kinase and glycolipid metabolism-related genes by insulin and glucagon in blunt snout bream (Megalobrama amblycephala): a comparative study
Transcriptional regulation of the AMP-activated protein kinase and glycolipid metabolism-related genes by insulin and glucagon in blunt snout bream (Megalobrama amblycephala): a comparative study
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团头鲂 (Megalobrama amblycephala) 中胰岛素和胰高血糖素对 AMP 激活蛋白激酶和糖脂代谢相关基因的转录调节：一项比较研究
DOI：
10.1016/j.aquaculture.2019.734553
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发表时间：
2019
期刊：
影响因子：
4.5
通讯作者：
Li Xiang-Fei
中科院分区：
文献类型：
--
作者：
Shi Hua-Juan;Liu Wen-Bin;Xu Chao;Zhang Li;Liu Jie;Zhang Ding-Dong;Zhang Ling;Li Xiang-Fei
研究了胰岛素和胰高血糖素对团头鲂（Megalobrama amblycephala）糖脂代谢的影响。鱼（平均重量49.5 ± 0.8 g）分别腹腔注射0.9%盐水溶液、牛胰岛素（0.052 mg/kg体重）和牛胰高血糖素（0.075 mg/kg体重）。然后，在注射后0、1、2、4、8和12 h取样。结果表明，胰岛素负荷可使血糖水平显著下降，1h时降至最低，而胰高血糖素负荷后血糖水平显著下降，4 h时达到最高。此后，血糖水平逐渐恢复至正常值。血浆甘油三酯（TG）水平显着下降，在第一个2小时后胰岛素注射。2 ~ 4 h明显升高，逐渐恢复正常。胰高血糖素给药显著降低血浆TG水平，在注射后8 h发现最低值。然后，它显着增加，并返回到基础值与进一步增加的时间。胰岛素注射导致肝糖原和脂质含量显著增加，分别在2和1 h达到峰值。然而，胰高血糖素注射诱导这两个参数的显着降低。随后，它们逐渐恢复到正常值。此外，胰岛素负荷后4 h内AMPKα1、AMPKα2、PEPCK、FBPase、G6 pase、PPARα、CPT Ⅰ和ACO的转录水平显著降低。而GLUT 2、GK、PK、SREBP 1、FAS和ACCα在2-4 h内均显著升高。注射胰高血糖素后，AMPKα1、AMPKα2、PEPCK、FBPase、G6 pase、PPARα、CPT I和ACO的转录值在前8 h内均显著升高，而其余指标在前4 h内则相反。外源性胰岛素和胰高血糖素显著影响团头鲂的糖脂代谢。注射胰岛素可显著降低血糖水平，但激活AMPK，从而促进葡萄糖转运、糖原生成、糖原生成和脂肪生成，同时抑制脂肪酸生成和脂肪酸氧化，而胰高血糖素负荷后则相反。
In this study, the effects of insulin and glucagon administration on the glycolipid metabolism of blunt snout bream (Megalobrama amblycephala) were investigated. Fish (average weight 49.5 ± 0.8 g) were intraperitoneally injected with a 0.9% saline solution, a bovine insulin (0.052 mg/kg body weight) and a bovine glucagon (0.075 mg/kg body weight), respectively. Then, samples were taken at 0, 1, 2, 4, 8 and 12 h post injection. The results showed that insulin load induced a significant decrease of plasma glucose level with the minimum observed at 1 h, while the opposite was true after glucagon injection (the highest value was attained at 4 h). After that, plasma glucose levels gradually returned to the normal value. Plasma triglycerides (TG) levels significantly declined during the first 2 h after insulin injection. Then, it increased significantly from 2 to 4 h, and gradually returned to the normal value. The glucagon administration significantly decreased plasma TG levels with the minimum value found at 8 h post-injection. Then, it significantly increased and returned to the basal value with further increasing times. Insulin injection resulted in a significantly increased liver glycogen and lipid contents, which peaked at 2 and 1 h, respectively. However, glucagon injection induced a significant decrease of both parameters. Subsequently, they gradually returned to the normal values. Besides, insulin load significantly decreased the transcriptional levels of AMPKα1, AMPKα2, PEPCK, FBPase, G6pase, PPARα, CPT Ⅰ and ACO during the first 4 h. However, those of GLUT2, GK, PK, SREBP1, FAS and ACCα all increased significantly during the 2–4 h. After the glucagon injection, the transcriptional values of AMPKα1, AMPKα2, PEPCK, FBPase, G6pase, PPARα, CPT I and ACO all increased significantly during the first 8 h, whereas the opposite was true for the remaining indicators during the first 4 h. Overall, exogenous insulin and glucagon significantly affected the glycolipid metabolism ofMegalobrama amblycephala. Insulin injection significantly decreased plasma glucose level, but activated AMPK, thus promoting glucose transportation, glycogenesis, glycogenesis and lipogenesis coupled with the depression of gluconeogenesis and fatty acid oxidation, whereas the opposite was true after the glucagon load.