Electrophysiologic and morphologic effects of ophthalmic preparations on rabbit cornea epithelium.
Electrophysiologic and morphologic effects of ophthalmic preparations on rabbit cornea epithelium.
复制标题
眼科制剂对兔角膜上皮的电生理和形态学影响。
DOI：
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发表时间：
1977
影响因子：
4.4
通讯作者：
S. Klyce
中科院分区：
文献类型：
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作者：
N. Burstein;S. Klyce
采用连续电生理监测和扫描电子显微镜固定的方法，研究了几种眼用制剂对离体兔角膜的影响。苯扎氯铵(0.001%)、硫柳汞(0.0004%)和两性霉素B(0.0025%)都短暂地增加了离子转运，然后大大降低了上皮阻力。表面细胞层的严重破坏与阻力的降低同时发生。硝酸银(0.00017%)促进细胞转运，并伴有较少的形态损伤。丁卡因(0.05%)扰乱了上皮功能，并导致几个细胞层脱落。氯丁醇(0.1%)使鳞状细胞层几乎完全消失。氯霉素、肾上腺素和匹罗卡品在完全临床浓度下产生轻微的结构和电生理变化。结论：眼用制剂中低浓度的防腐剂破坏了角膜上皮的屏障和运输特性。
The effects of several components of ophthalmic preparations on isolated rabbit cornea were studied by continuous electrophysiologic monitoring followed by fixation for scanning electron microscopy (SEM). Benzalkonium chloride (0.001 percent), thimerosal (0.0004 percent), and amphotericin B (0.0025 percent) all briefly increased ion transport, then greatly decreased epithelial resistance. Severe disruption of surface cell layers occurred simultaneously with resistance decrease. Silver nitrate (0.00017 percent) stimulated transport with less accompanying morphologic damage. Tetracaine (0.05 percent) disrupted epithelial function and caused exfoliation of several cell layers. Chlorobutanol (0.1 percent) produced a nearly complete loss of the squamous cell layer. Chloramphenicol, epinephrine, and pilocarpine produced minor changes in structure and electrophysiology at full clinical concentration. It was concluded that low concentrations of preservatives in ophthalmic preparations disrupt the barrier and transport properties of the corneal epithelium.