PATHOGENESIS OF VIRULENT AND AVIRULENT AVIAN INFECTIOUS BRONCHITIS VIRUS
PATHOGENESIS OF VIRULENT AND AVIRULENT AVIAN INFECTIOUS BRONCHITIS VIRUS
复制标题
DOI：
10.1007/bf01242547
复制
发表时间：
1973-01-01
期刊：
影响因子：
--
通讯作者：
LUKERT, PD
中科院分区：
文献类型：
--
作者：
GEILHAUSEN, HE;LIGON, FB;LUKERT, PD
本研究比较了鸡传染性支气管炎病毒（IBV）强毒株和无毒株的感染情况。 60 只 3 周龄鸡被气溶胶感染 IBV 强毒株，60 只鸡被无毒株感染。气管印模涂片和低温切片的免疫荧光染色显示，剧毒IBV感染并破坏了气管内壁的纤毛上皮细胞。然后病毒定位于上皮下细胞并可在 21 天内检测到。无毒力的IBV仅感染少数纤毛上皮细胞并且上皮没有明显脱落。无毒力 IBV 与强毒力一样，定位于上皮下细胞，并可在 24 天内检测到。通过病毒中和滴度测量，感染后 19 天对强毒力和无毒力 IBV 的免疫反应相似。此后，反应出现分歧，强毒病毒诱导抗体滴度上升，而无毒病毒则抗体滴度下降。
This study compared the infections of chickens with virulent and avirulent strains of avian infectious bronchitis virus (IBV). Sixty 3-week-old chickens were infected by aerosol with a virulent strain of IBV and 60 with an avirulent strain. Immunofluorescent staining of impression smears and cryostat sections of the trachea revealed that the virulent IBV infected and destroyed the ciliated epithelial cells lining the trachea. The virus then became localized in subepithelial cells and was detectable for 21 days. The avirulent IBV infected only a few ciliated epithelial cells and there was no significant desquamation of the epithelium. The avirulent IBV, like the virulent, became localized in subepithelial cells and was detectable for 24 days. The immunologie response to virulent and avirulent IBV, as measured by virus neutralization titers, was similar at 19 days post infection. After this time, there was a divergence of the responses, with the virulent virus inducing ascending antibody titers while the antibody titers descended with the avirulent virus.