In vitro biocompatibility of oxirane/polyol dental composites with promising physical properties
In vitro biocompatibility of oxirane/polyol dental composites with promising physical properties
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DOI：
10.1016/s0109-5641(01)00071-9
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发表时间：
2002-07-01
期刊：
影响因子：
5
通讯作者：
Yourtee, DM
中科院分区：
文献类型：
--
作者：
Eick, JD;Kostoryz, EL;Yourtee, DM
目的：CyracureTM UVR-6105与pTHF-250的可见光固化环氧乙烷/多元醇树脂先前已显示可用于牙科复合材料的开发。将该环氧乙烷/多元醇（4016）与其它环氧乙烷组合配制成含有72.9-74.9%石英填料的复合材料（4016 E、4016 G和4016 GB）。该研究的主要目的是评估一些的物理性能和生物相容性的composities.Results：复合材料的PhotoDSC分析表明，Z100（31 J/g）的physiology值的两倍。复合材料4016 E和4016 G的压缩强度与Z100相似（337+/-35 Mpa），P > 0.05。含有Epon（TM）825环氧乙烷（E）的复合材料4016 E和含有Araldite（TM）戈伊281环氧乙烷（G）的复合材料4016 G的圆盘无细胞毒性（-），而含有G和Ebecryl（TM）1830（13）的复合材料4016 GB在琼脂扩散试验中对L929细胞具有轻度（+）细胞毒性。在MTT试验中，4016 GB复合材料的7天浸提液具有细胞毒性，而4016 E和4016 G的浸提液对L929细胞的细胞毒性较小。在37 ℃下，将复合材料（3 cm 2表面积/ml）在丙酮或乙醇/盐水（1：20）中孵育7天，获得浸提液。所有复合浸提液对艾姆斯菌株TA 100、TA 98、TA 97 a和TA 1535均无致突变性。复合材料4016 GB的总体结果表明，可沥滤组分具有细胞毒性，但无致突变性。除环氧乙烷组分G和E外，环氧乙烷Cyracure（TM）LTVR-6105和其他组分均无致突变性。根据细胞毒性研究，光引发剂SarcatTM CD 1012是最具细胞毒性（TC 50 = 14 μ M）的组分。构成部分G（TC 50 = 17 μ M），E（TC 50 = 50 μ M）和B（TC 50 = 151 μ M），（p < 0.05）比Cyracure（TM）UVR-6105更有细胞毒性（1488 μ M）和多元醇pTHF-250（TC 50 = 6072 μ M）。用复合材料4016 G和4016 E获得的有利结果表明，合适的环氧乙烷/可以设计和优化多元醇配方以开发具有可接受的机械性能和生物相容性的牙科复合材料。然而，在得出关于环氧乙烷组分可溶解性的最终结论之前，需要对从较长孵育期获得的浸提液进行沥滤物分析。(C)2002年牙科材料学院。由Elsevier Science Ltd.出版，版权所有。
Objectives: Visible light cure oxirane/polyol resins of Cyracure(TM) UVR-6105 with pTHF-250 has been previously shown useful for development of dental composites. This oxirane/polyol (4016) in combination with other oxiranes were formulated into composites (4016E, 4016G and 4016GB) containing 72.9-74.9% quartz filler. The main objective of the study was to evaluate some of the physical properties and the biocompatibility of the composites.Results: PhotoDSC analysis of composites demonstrated twice the enthalphy values of Z100 (31 J/g). Composites 4016E and 4016G showed compressive strengths similar to Z100 (337+/-35 Mpa), P > 0.05. Discs of composite 4016E, containing Epon(TM) 825 oxirane (E), and composite 4016G containing Araldite(TM) GY 281 oxirane (G) were non-cytotoxic (-) while the composite 4016GB, containing G and Ebecryl(TM) 1830 (13), was mildly (+) cytotoxic to L929 cells in the agar diffusion assay. Seven day extracts of 4016GB composite were cytotoxic while extracts of 4016E and 4016G were less cytotoxic to L929 cells in the MTT assay. Extracts were obtained from 7 day incubations of composite (3 cm 2 surface area/ml) in acetone or ethanol/saline (1:20) at 37degreesC. All composite extracts were non-mutagenic to Ames strains TA100, TA98, TA97a and TA1535. The overall results with composite 4016GB suggest that leachable components were cytotoxic but non-mutagenic. With the exception of oxirane components, G and E, the oxirane Cyracure(TM) LTVR-6105 and other components were non-mutagenic. From cytotoxicity studies, the photoinitiator, Sarcat(TM) CD 1012, was the most cytotoxic (TC50 = 14 muM) component. Components G (TC50 = 17 muM), E (TC50 = 50 muM) and B (TC50 = 151 muM) were significantly (p < 0.05) more cytotoxic than Cyracure(TM) UVR-6105 (1488 muM) and the polyol, pTHF-250 (TC50 = 6072 muM).Significance: Favorable results obtained with composites 4016G and 4016E indicates that suitable oxirane/polyol formulations can be designed and optimized for development of dental composites with acceptable mechanical properties and biocompatibility. However, leachable analysis of extracts obtained from longer incubation periods is needed before final conclusions could be drawn about the leachability of oxirane components. (C) 2002 Academy of Dental Materials. Published by Elsevier Science Ltd. All rights reserved.