A STATIONARY-PHASE FOR OPEN-TUBULAR LIQUID-CHROMATOGRAPHY AND ELECTROCHROMATOGRAPHY USING SOL-GEL TECHNOLOGY
A STATIONARY-PHASE FOR OPEN-TUBULAR LIQUID-CHROMATOGRAPHY AND ELECTROCHROMATOGRAPHY USING SOL-GEL TECHNOLOGY
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DOI：
10.1021/ac00111a003
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发表时间：
1995-08-01
影响因子：
7.4
通讯作者：
COLON, LA
中科院分区：
文献类型：
--
作者：
GUO, Y;COLON, LA
采用溶胶-凝胶法制备了一种有机-无机杂化材料，并将其作为薄玻璃膜浇铸在熔融石英毛细管柱内壁上。毛细管中的薄膜作为反相开管液相色谱（OTLC）和开管电色谱（OTEC）的固定相。固定相提供高表面积，从而改善相比。通过在单一程序中附着固定相和增加表面积，避免了多个制备步骤。通过调节原溶胶-凝胶溶液中单体前驱体的比例，控制固定相的保留特性。该方法便于OTLC和OTEC的色谱柱制备。用这种方法制备的毛细管柱的保留特性优于常规制备的毛细管柱。用多环芳烃评价固定相；在实验条件下，OTLC和OTEC的效率分别为204 000 ~ 285 000和28 000 ~ 50 000片/m。溶胶-凝胶衍生的固定相在酸性和碱性条件下都是稳定的。
An organic-inorganic hybrid material was fabricated by the sol-gel method and cast as a thin glass film onto the inner walls of fused silica capillary columns. The thin film in the capillaries functioned as the stationary phase for reversed phase open tubular liquid chromatography (OTLC) and open tubular electrochromatography (OTEC). The stationary phase provides high surface area and thus an improved phase ratio. Multiple preparation steps are avoided by attaching the stationary phase and increasing the surface area in a single procedure. By adjusting the ratio of the monomeric precursors in the original sol-gel solution, the retentive characteristics of the stationary phase are controlled. This new approach facilitates column preparation for OTLC and OTEC. Capillary columns prepared in this manner showed retention characteristics superior to conventionally prepared ones. Polycyclic aromatic hydrocarbons were used to evaluate the stationary phase; under the experimental conditions used, efficiencies of 204 000-285 000 and 280 000-500 000 plates/m were observed in OTLC and OTEC, respectively. The sol-gel-derived stationary phase was shown to be stable under acidic and basic conditions.