Stochastic Optical Reconstruction Microscopy (STORM).
Stochastic Optical Reconstruction Microscopy (STORM).
复制标题
随机光学重建显微镜（STORM）。
DOI：
10.1002/cpcy.23
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发表时间：
2017
影响因子：
--
通讯作者：
Liu,Yang
中科院分区：
文献类型：
--
作者：
Xu,Jianquan;Ma,Hongqiang;Liu,Yang
超分辨率（SR）荧光显微镜是一类空间分辨率低于衍射极限的光学显微镜技术，它彻底改变了我们研究生物学的方式，这一点得到了2014年诺贝尔化学奖的认可。随机光学重建显微术（STORM）是一种基于单分子定位原理的广泛应用的超分辨率成像技术。STORM通常可实现20至30 nm的空间分辨率，与传统光学显微镜相比提高了10倍。在所有SR技术中，STORM提供了高空间分辨率，具有简单的光学仪器和标准的有机荧光染料，但由于不适当的样品制备或成像条件，它也容易出现图像伪影和图像分辨率下降。它需要仔细优化所有三个方面-样品制备，图像采集和图像重建-以确保高质量的STORM图像，这将在本单元中广泛讨论。John Wiley & Sons，Inc.
Super‐resolution (SR) fluorescence microscopy, a class of optical microscopy techniques at a spatial resolution below the diffraction limit, has revolutionized the way we study biology, as recognized by the Nobel Prize in Chemistry in 2014. Stochastic optical reconstruction microscopy (STORM), a widely used SR technique, is based on the principle of single molecule localization. STORM routinely achieves a spatial resolution of 20 to 30 nm, a ten‐fold improvement compared to conventional optical microscopy. Among all SR techniques, STORM offers a high spatial resolution with simple optical instrumentation and standard organic fluorescent dyes, but it is also prone to image artifacts and degraded image resolution due to improper sample preparation or imaging conditions. It requires careful optimization of all three aspects—sample preparation, image acquisition, and image reconstruction—to ensure a high‐quality STORM image, which will be extensively discussed in this unit. © 2017 by John Wiley & Sons, Inc.