Inhibition of laryngeal cancer cell activity by pterostilbene via modulating AKT/mTOR signal pathway and related mechanisms
Inhibition of laryngeal cancer cell activity by pterostilbene via modulating AKT/mTOR signal pathway and related mechanisms
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发表时间：
2018
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通讯作者：
Ying Yang;Juyi Wen;Jingjiao Li;Yuanyuan Zhang;Henghu Fang;Sudong Wu;Tingjun Dong;Panpan;Gan;C. Cai
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作者：
Ying Yang;Juyi Wen;Jingjiao Li;Yuanyuan Zhang;Henghu Fang;Sudong Wu;Tingjun Dong;Panpan;Gan;C. Cai
AKT/mTOR signal pathway participates in growth, proliferation and invasion of tumor cells, and is correlated with onset and occurrence of laryngeal cancer. Resveratrol can modulate proliferation of laryngeal cancer cells. Pterostilbene, as one homolog of resveratrol, has more potent effects for inhibiting tumor cell occurrence and progression, but leaving its role and mechanism for laryngeal cancer cells unillustrated yet. This study investigated the effect of pterostilbene on laryngeal cancer cells and AKT/mTOR signal pathway. Laryngeal cancer cell line Hep-2 was treated with 100 μmol/L or 200 μmol/L pterostilbene, followed by MTT assay for cell proliferation, caspase-3 activity assay for cell apoptosis, and cell invasion assay. Western blot was used to test the change of AKT protein phosphorylation. Real-time PCR was adopted to describe mTOR expression. Pterostilbene significantly inhibited Hep-2 cell proliferation and invasion, and enhanced Caspase-3 activity (p<0.05 compared to control group). 200 μmol/L pterostilbene had more potent effects than 100 μmol/L group. Pterostilbene also suppressed mTOR mRNA of pharyngeal cancer cells, and decreased phosphorylation level of AKT (p<0.05 compared to control group). Pterostilbene can regulate proliferation, apoptosis and invasion of pharyngeal cancer cells via inhibiting AKT/mTOR signal pathway.
