In vitro activity of five flavones from Scutellaria baicalensisin combination with Cefazolin against methicillin resistant Staphylococcus aureus (MRSA)
In vitro activity of five flavones from Scutellaria baicalensisin combination with Cefazolin against methicillin resistant Staphylococcus aureus (MRSA)
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DOI：
10.1007/s00044-016-1685-9
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发表时间：
2016-10-01
影响因子：
2.6
通讯作者：
Jiang, Liyin
中科院分区：
文献类型：
--
作者：
Qiu, Fan;Meng, Li;Jiang, Liyin
本研究的目的是研究黄芩素、黄芩苷、汉黄素、汉黄酮苷和头孢唑林联合应用对耐甲氧西林金黄色葡萄球菌的体外抗菌活性。用棋盘法评价黄酮类药物与头孢唑啉的联合作用，得到分数抑菌浓度指数。比较了黄芩素和黄芩苷对耐甲氧西林金黄色葡萄球菌的抗菌作用，并用时间-杀灭曲线描述了其抗菌过程。结果表明，黄芩中的5种黄酮类成分均能不同程度地增强头孢唑林对耐甲氧西林金黄色葡萄球菌的抗药性，其中黄芩素和黄芩苷的作用更强。黄芩素与抗生素配伍的最低抑菌浓度较低，抑菌作用快于黄芩苷。黄芩素和黄芩苷在时间杀灭曲线的第一时间段出现不同的屈曲，显示出不同的作用速度。综上所述，本研究表明这些黄酮类化合物能够恢复头孢唑林对耐甲氧西林金黄色葡萄球菌的抗菌活性。
The aim of this study was to investigate the in vitro activities of baicalein, baicalin, wogonin, wogonoside and oroxylin A in combination with cefazolin against Methicillin-resistant Staphylococcus aureus. The combination effect of flavones and cefazolin was evaluated by checkerboard method to obtain a fractional inhibitory concentration index. The anti-Methicillin-resistant Staphylococcus aureus effect of baicalein and baicalin were compared and the antibacterial process was presented by time-kill curves. It was found that these five flavones from Scutellaria baicalensis Georgi could strengthen the anti-Methicillin-resistant Staphylococcus aureus effect of cefazolin to varying degrees, and baicalein and baicalin have stronger effect. Baicalein have the lower minimum inhibitory concentration in combination with antibiotic and quicker antibacterial effect than that of baicalin. There was different buckling in the first time interval of time kill curve between baicalein and baicalin showing the different speed of action. In conclusion, this study showed these flavones could restore the activity of cefazolin against Methicillin-resistant Staphylococcus aureus.