Cytokeratin expression in palatal and marginal mucosa of cleft palate patients
Cytokeratin expression in palatal and marginal mucosa of cleft palate patients
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DOI：
10.1016/j.archoralbio.2006.01.004
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发表时间：
2006-07-01
影响因子：
3
通讯作者：
den Hoff, Johannes. W. Von
中科院分区：
文献类型：
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作者：
Guo, Ji H.;Maltha, Jaap C.;den Hoff, Johannes. W. Von
目的：腭裂边缘是一个独特的解剖部位，因为腭粘膜与鼻或鼻咽粘膜是连续的。本研究的目的是比较细胞角蛋白和基底膜成分在腭裂区粘膜的表达模式。设计：对5例腭裂初次手术闭合患者进行硬腭黏膜和软腭裂缘活检。对组织进行苏木精-伊红染色和免疫组织化学处理。用抗细胞角蛋白（CK） 4、7、8、10、13、16和18的抗体，以及基膜成分硫酸肝素（HS）和IV型胶原（CIV）的抗体进行免疫染色。结果：鼻咽上皮较软腭粘膜薄，乳头间脊较少。鼻咽上皮呈层状，但表达单一上皮的角蛋白（ck7、8和18）。在向软腭上皮过渡时，表达模式突然转变为典型的非角化层状上皮（ck4,13）。硬腭的上皮是完全分化、角化和分层的上皮（CK 10,16）。鼻咽上皮基底膜较薄，可能与炎症细胞丰富有关。结论：腭裂周围有三种不同类型的上皮。从口腔侧到鼻咽侧的上皮表型有一个突变。(c) 2006 Elsevier Ltd.版权所有。
Objective: The margin of a palatal cleft is a unique anatomical site since the palatal mucosa is continuous with the nasal or nasopharyngeal mucosa. The aim of this study was to compare the expression patterns of cytokeratins and basal membrane components of the mucosa in the area of the cleft.Design: Biopsies from the mucosa of the hard palate and from the cleft margin in the soft palate were obtained from five patients during the primary surgical closure of the cleft. The tissues were processed for haematoxylin-eosin staining and for immuno-histochemistry. Antibodies against the cytokeratins (CK) 4, 7, 8, 10, 13, 16 and 18, and the basal membrane components heparan sulphate (HS) and collagen type IV (CIV) were used for immunostaining.Results: The nasopharyngeal epithelium was thinner than the epithelium of the soft palatal mucosa, and showed less interpapillary ridges. The nasopharyngeal epithelium was stratified but expressed the keratins of a simple epithelium (CK 7, 8 and 18). The expression pattern abruptly changed into that of a typical non-keratinized stratified epithelium (CK 4, 13) at the transition to the soft palatal epithelium. The epithelium of the hard palate was a fully differentiated, keratinized and stratified epithelium (CK 10, 16). The basal membrane was thinner in the nasopharyngeal epithelium, which might be related to the presence of abundant inflammatory cells.Conclusion: The area around the palatal cleft showed three different types of epithelium. There was an abrupt transition in phenotype of the epithelium from the oral side to the nasopharyngeal side. (c) 2006 Elsevier Ltd. All rights reserved.