Inhibition of Leishmania (Leishmania) amazonensis growth and infectivity by aureobasidin A.

Inhibition of Leishmania (Leishmania) amazonensis growth and infectivity by aureobasidin A.
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aureobasidin A 抑制亚马逊利什曼原虫 (Leishmania) 的生长和感染性。

DOI：
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发表时间：
2007
期刊：
Journal of Antimicrobial Chemotherapy
影响因子：
5.2
通讯作者：
A. H. Straus
A. H. Straus
中科院分区：
医学2区
文献类型：
--
作者：
A. K. Tanaka;V. B. Valero;Hélio K. Takahashi;A. H. Straus

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目标 研究肌醇磷酸神经酰胺（IPC）合成酶抑制剂金梗霉素A对利什曼原虫生长和感染性的影响。 方法 测定了金芽孢杆菌素A对以下方面的影响：（i）无菌培养物中的前鞭毛体生长;（ii）巨噬细胞单层中的前鞭毛体感染性;（iii）BALB/c小鼠足垫病变的发展;（iv）无鞭毛体向前鞭毛体的分化。 结果 Aureobasidin A（20 μ M）在无菌培养物中抑制90%的利什曼原虫（利什曼原虫）亚马逊前鞭毛体生长，但寄生虫保持活力，即从培养基中除去Aureobasidin A后生长曲线恢复正常。用MTT法测得金霉素A对L.（L.）亚马逊利什曼原虫（Leishmaniasmajor）为12.6 μ M，巴西利什曼原虫（Leishmaniasbraziliensis）为13.7 μ M。当L.（L.）将用金杆菌素A预处理的亚马逊前鞭毛体接种到BALB/c小鼠足垫中。当将金芽孢杆菌素A添加到感染无鞭毛体的培养的巨噬细胞中时，感染的巨噬细胞的数量减少> 90%。 结论 Aureobasidin A是研究利什曼原虫属中脂质代谢抑制作用的有趣药理学工具。
OBJECTIVES To study the effect of aureobasidin A, an inhibitor of inositol phosphorylceramide (IPC) synthase, on Leishmania growth and infectivity. METHODS Effects of aureobasidin A were determined for: (i) promastigote growth in axenic culture; (ii) promastigote infectivity in macrophage monolayers; (iii) development of footpad lesions in BALB/c mice; (iv) differentiation of amastigotes into promastigotes. RESULTS Aureobasidin A (20 microM) inhibited 90% of Leishmania (Leishmania) amazonensis promastigote growth in axenic culture, but the parasites remained viable, i.e. growth curves returned to normal after aureobasidin A was removed from culture medium. The aureobasidin A IC50 was determined by MTT assay as 4.1 microM for L. (L.) amazonensis promastigotes, 12.6 microM for Leishmania (Leishmania) major and 13.7 microM for Leishmania (Viannia) braziliensis. There was a significant delay in infection when L. (L.) amazonensis promastigotes pre-treated with aureobasidin A were inoculated into BALB/c mouse footpads. When aureobasidin A was added to cultured macrophages infected with amastigotes, the number of infected macrophages was reduced by >90%. CONCLUSIONS Aureobasidin A is an interesting pharmacological tool to investigate the effect of lipid metabolism inhibition in Leishmania spp.
Stage-specific glycosphingolipids from amastigote forms of Leishmania (L.) amazonensis. Immunogenicity and role in parasite binding and invasion of macrophages.
来自亚马逊利什曼原虫（L.）无鞭毛体形式的阶段特异性糖鞘脂。
DOI： --
发表时间： 1993
期刊： The Journal of biological chemistry
影响因子： --
作者：
Straus,AH;Levery,SB;Jasiulionis,MG;Salyan,ME;Steele,SJ;Travassos,LR;Hakomori,S;Takahashi,HK
通讯作者： Takahashi,HK