A simple Na2SO3 feeding method for KLa measurement in large‐scale fermentors
A simple Na2SO3 feeding method for KLa measurement in large‐scale fermentors
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DOI：
10.1002/bit.260290810
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发表时间：
1987-06
影响因子：
3.8
通讯作者：
Y. Imai;H. Takei;M. Matsumura
中科院分区：
文献类型：
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作者：
Y. Imai;H. Takei;M. Matsumura
提出了一种在大型发酵罐中测定KLa的简便方法。该方法基于在稳态条件下溶解氧浓度的测量，所述稳态条件由亚硫酸根离子进料和化学氧化速率的等效性建立。该方法具有以下优点：它是一种稳态方法，因此不必考虑溶解氧探针的响应滞后和由于发酵罐中气相混合引起的响应滞后。在该测量系统中，排出气体的氧含量与鼓泡空气的氧含量几乎相同。因此，可以使用喷射空气的氧分压来计算氧转移的驱动力。不需要关于亚硫酸盐氧化动力学的详细信息，因为稳定状态下的溶解氧浓度不受亚硫酸盐氧化速率的影响。KLa的测量在150秒的短时间内完成，即使在发酵罐的体积为10 m3。由于快速测量，试验发酵罐中Na2SO4的积累量非常小，因此通过该方法获得的KLa值适用于无电解质系统。此外，由于盐浓度低，我们可以将发酵罐中的废液排放到排水管中，而无需任何预处理。
A simple and convenient method for measuring KLa in large‐scale fermentors was proposed. This method was based on the measurement of the dissolved oxygen concentration under steady state conditions established by an equivalency of the sulfite ion feed and chemical oxidation rates. This method had the following advantages: It was a steady state method, and so it was not necessary to consider the response lag of a dissolved oxygen probe and the response lag due to gas phase mixing in fermentors. The oxygen content of the effluent gas in this measuring system was nearly the same as that of the sparged air. Therefore, it was possible to use the oxygen partial pressure of the sparged air for the calculation of the driving force of oxygen transfer. The detailed information on the kinetics of sulfite oxidation was not necessary, because the dissolved oxygen concentration in steady state was not influenced by sulfite oxidation rates. The KLa measurement was finished in as short a period as 150 s, even in a fermentor with a volume of 10 m3. Since the amount of Na2SO4 accumulation in the test fermentors was very small because of the quick measurement, the KLa values obtained by this method were applicable to the electrolyte‐free system. Furthermore, we could discharge the used liquid from the fermentors into a drain without any pretreatment due to the low salt concentration.