The post-transcriptional trans-acting regulator, TbZFP3, co-ordinates transmission-stage enriched mRNAs in Trypanosoma brucei.
The post-transcriptional trans-acting regulator, TbZFP3, co-ordinates transmission-stage enriched mRNAs in Trypanosoma brucei.
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DOI：
10.1093/nar/gkr1106
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发表时间：
2012-04
影响因子：
14.9
通讯作者：
Matthews KR
中科院分区：
文献类型：
--
作者：
Walrad PB;Capewell P;Fenn K;Matthews KR
转录后基因调控对真核生物的发育至关重要。这一点在锥虫寄生虫中尤为突出，锥虫的基因在多顺反电子阵列中共转录，但不一定共调控。小CCCH蛋白TbZFP3已被确定为布鲁氏锥虫原环素表面抗原表达的反式作用转录后调节剂。为了研究TbZFP3在寄生虫传播中的更广泛作用，对相关转录本进行了全球分析。对所选转录本的一个子集的检查显示，TbZFP3异位过表达的mRNA稳定增加了它们的丰度，这取决于CCCH锌指结构域的完整性。报告者实验表明，这种调节是通过两个目标转录本的3 ' -UTR序列介导的。tbzfp3选择的转录本群体的全球发育表达谱显示，它们在疟原虫的可传播矮株形式中显著富集。对TbZFP3mRNP选择的特定mrna的分析为具有协调寄生虫传播中重要基因的潜力的发育调节提供了证据。
Post-transcriptional gene regulation is essential to eukaryotic development. This is particularly emphasized in trypanosome parasites where genes are co-transcribed in polycistronic arrays but not necessarily co-regulated. The small CCCH protein, TbZFP3, has been identified as a trans-acting post-transcriptional regulator of Procyclin surface antigen expression in Trypanosoma brucei. To investigate the wider role of TbZFP3 in parasite transmission, a global analysis of associating transcripts was carried out. Examination of a subset of the selected transcripts revealed their increased abundance through mRNA stabilization upon TbZFP3 ectopic overexpression, dependent upon the integrity of the CCCH zinc finger domain. Reporter assays demonstrated that this regulation was mediated through 3′-UTR sequences for two target transcripts. Global developmental expression profiling of the cohort of TbZFP3-selected transcripts revealed their significant enrichment in transmissible stumpy forms of the parasite. This analysis of the specific mRNAs selected by the TbZFP3mRNP provides evidence for a developmental regulon with the potential to co-ordinate genes important in parasite transmission.
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