Sensitive detection of mercury and copper ions by fluorescent DNA/Ag nanoclusters in guanine-rich DNA hybridization
Sensitive detection of mercury and copper ions by fluorescent DNA/Ag nanoclusters in guanine-rich DNA hybridization
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富鸟嘌呤 DNA 杂交中荧光 DNA/Ag 纳米簇灵敏检测汞和铜离子
DOI：
10.1016/j.saa.2014.08.135
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发表时间：
2015-02-25
影响因子：
4.4
通讯作者：
Ding, Zhong-Tao
中科院分区：
文献类型：
--
作者：
Peng, Jun;Ling, Jian;Ding, Zhong-Tao
在这项工作中，我们设计了一种新的荧光寡核苷酸稳定银纳米簇（DNA/ agnc）探针，用于敏感检测汞和铜离子。该探针包含两个定制的DNA序列。一种是信号探针，包含用于agnc合成的富含胞嘧啶的序列模板和两端的连接序列。另一个是用于信号增强的富鸟嘌呤序列和与信号探针连接序列互补的连接序列。杂交后，基于DNA/ agnc在富鸟嘌呤DNA序列附近的荧光增强效应，杂交后的双链DNA/ agnc的荧光增强了200倍。双链DNA/AgNCs探针比单链DNA/AgNCs探针更亮、更稳定，更重要的是，它可以作为一种新型的荧光探针用于检测汞和铜离子。在6.0 ~ 160.0 nM和6 ~ 240 nM范围内的汞离子和铜离子均可线性检测，检出限分别为2.1 nM和3.4 nM。结果表明，该方法检测汞和铜离子的分析参数远优于单链DNA/ agnc。(C) 2014 Elsevier B.V.版权所有
In this work, we designed a new fluorescent oligonucleotides-stabilized silver nanoclusters (DNA/AgNCs) probe for sensitive detection of mercury and copper ions. This probe contains two tailored DNA sequence. One is a signal probe contains a cytosine-rich sequence template for AgNCs synthesis and link sequence at both ends. The other is a guanine-rich sequence for signal enhancement and link sequence complementary to the link sequence of the signal probe. After hybridization, the fluorescence of hybridized double-strand DNA/AgNCs is 200-fold enhanced based on the fluorescence enhancement effect of DNA/AgNCs in proximity of guanine-rich DNA sequence. The double-strand DNA/AgNCs probe is brighter and stable than that of single-strand DNA/AgNCs, and more importantly, can be used as novel fluorescent probes for detecting mercury and copper ions. Mercury and copper ions in the range of 6.0-160.0 and 6-240 nM, can be linearly detected with the detection limits of 2.1 and 3.4 nM, respectively. Our results indicated that the analytical parameters of the method for mercury and copper ions detection are much better than which using a single-strand DNA/AgNCs. (C) 2014 Elsevier B.V. All rights reserved.