Phosphoinositide metabolism at fertilization of sea urchin eggs measured with a GFP-probe.
Phosphoinositide metabolism at fertilization of sea urchin eggs measured with a GFP-probe.
复制标题
用 GFP 探针测量海胆卵受精时的磷酸肌醇代谢。
DOI：
10.1111/j.1440-169x.2004.00758.x
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Epel,David
中科院分区：
文献类型：
--
作者：
Thaler,CatherineD;Kuo,RichardC;Patton,Chris;Preston,ChristinaM;Yagisawa,Hitoshi;Epel,David
Fertilization elicits a dramatic, transient rise in Ca2+within the egg which is an essential component of egg activation and consequent initiation of development. In the sea urchin egg, three distinct Ca2+stores have been identified which could, either individually or in combination, initiate Ca2+release at fertilization. Inositol 1,4,5‐trisphosphate (IP3) production by phospholipase C (PLC) has been suggested as the singular signal in initiating the Ca2+transient. Other studies indicate that Ca2+stores gated by cyclic adenosine diphosphate ribose (cADPR) or nicotinic acid adenine dinucleotide phosphate (NAADP) are also necessary. We have examined the temporal relationship between the Ca2+rise and IP3production at fertilizationin vivowithin individual eggs using a green fluorescent protein (GFP) coupled to a pleckstrin homology (PH) domain that can detect changes in IP3. Translocation of the probe occurred after the Ca2+rise was initiated. Earlier, and possibly smaller, IP3changes could not be excluded due to limitations in probe sensitivity. High IP3levels are maintained during the decline in cytoplasmic Ca2+, suggesting that later IP3metabolism might not be related to regulation of Ca2+, but may function to modulate other PIP2regulated events such as actin polymerization or reflect other novel phosphoinositide signaling pathways.
