DISTINCT NUCLEAR AND SPINDLE POLE BODY POPULATIONS OF CYCLIN-CDC2 IN FISSION YEAST
DISTINCT NUCLEAR AND SPINDLE POLE BODY POPULATIONS OF CYCLIN-CDC2 IN FISSION YEAST
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DOI：
10.1038/347680a0
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发表时间：
1990-10-18
期刊：
影响因子：
64.8
通讯作者：
HYAMS, JS
中科院分区：
文献类型：
--
作者：
ALFA, CE;DUCOMMUN, B;HYAMS, JS
细胞周期蛋白作为dc 2基因编码的蛋白激酶的亚单位，是真核细胞周期1 -3的主要调控元件。裂殖酵母裂殖酵母具有B型细胞周期蛋白，其是由dc 13基因4 -6编码的核蛋白7，8。在这里，我们证明了存在两个空间上不同的cdc 13细胞周期蛋白群体的细胞核中的S。粟酒裂殖子，其中之一与有丝分裂纺锤体极有关。这两个群体都与dc 2基因产物（p34 cdc 2）共定位。用抗微管药物噻苯达唑处理细胞可以阻止细胞周期蛋白的降解，阻断酪氨酸去磷酸化和cdc 2的激活。这些结果表明，cdc 2-细胞周期蛋白复合物在有丝分裂纺锤体形成的启动中起着关键的调节作用，并且cdc 2激活也需要有丝分裂微管功能。
CYCLINS, as subunits of the protein kinase encoded by thecdc2gene are major controlling elements of the eukaryotic cell cycle1–3. The fission yeastSchizosaccharomyces pombehas a B-type cyclin, which is a nuclear protein7,8encoded by thecdc13gene4–6. Here we demonstrate the presence of two spatially distinct cdc13 cyclin populations in the nucleus ofS. pombe, one of which is associated with the mitotic spindle poles. Both populations colocalize with the product of thecdc2gene (p34cdc2). Treatment of cells with the antimicrotubule drug thiabendazole prevents cyclin degradation and blocks the tyrosine dephosphorylation and activation of cdc2. These results suggest a key regulatory role of the cdc2-cyclin complex in the initiation of mitotic spindle formation and also that mitotic microtubule function is required for cdc2 activation.