A database for regional gene expression in the human brain
A database for regional gene expression in the human brain
复制标题
DOI：
10.1016/s1567-133x(01)00006-0
复制
发表时间：
2001-08-01
影响因子：
1.2
通讯作者：
Riggins, Gregory J.
中科院分区：
文献类型：
--
作者：
Siu, I.-Mei;Lal, Anita;Riggins, Gregory J.
基因表达水平的变化在各种神经疾病中得到了广泛的研究，但很少有研究试图描述正常大脑不同区域的全基因组基因表达模式。一种用于量化转录水平的灵敏方法。基因表达系列分析(SAGE)用于检测快速尸检获得的同一正常脑中白质、丘脑和小脑的表达水平。这些SAGE文库的完整数据集发布在癌症基因组解剖项目赞助的SAGE地图网站(http://www.ncbi.nlm.nih.gov/SAGE/(3)))上，可以在该网站上进行文库比较，或下载数据用于本地分析。用实时荧光定量RT-PCR证实了神经源性分化1(小脑)、可卡因和安非他明调节的转录本(丘脑)和神经颗粒素(白质)等几个区域特异性基因的表达。对数据的进一步信息学分析得出了一份大脑特有基因的清单。通过这种分析形成的数据库为研究正常大脑区域特定功能涉及的基因的表达状态提供了一种手段。这些正常的脑基因表达水平也可用于与病理表达水平的比较，以及在制定治疗策略之前确定正常成人大脑中的基因表达的敏感手段。
Alterations in gene expression levels have been widely studied for various neurological diseases, but few studies have sought to characterize genome-wide gene expression patterns from various regions of the normal brain. A sensitive method for quantifying transcript levels. Serial Analysis of Gene Expression (SAGE), was used to assay expression levels in white matter, thalamus, and cerebellum from the same normal brain, obtained by rapid autopsy. The complete dataset for these SAGE libraries are posted on the Cancer Genome Anatomy Project sponsored SAGEmap website (http://www.ncbi.nlm.nih.gov/SAGE/(3)) where library comparisons can be made, or the data downloaded for local analysis. The expression of several region-specific genes - neurogenic differentiation 1 (cerebellum), cocaine- and amphetamine-regulated transcript (thalamus), and neurogranin (white matter) - was confirmed using quantitative fluorescent real-time RT-PCR. Further informatics analysis of the data yielded a list of brain-specific genes. The database formed by this analysis provides a means to investigate the expression status of genes involved in the region specific functions of the normal brain. These normal brain gene expression levels also are useful for comparison to pathological expression levels and a sensitive means to determine gene expression in a normal adult brain prior to formulating therapeutic strategies.