Limitations to oxygen diffusion and equilibration in in vitro cell exposure systems in hyperoxia and hypoxia
Limitations to oxygen diffusion and equilibration in in vitro cell exposure systems in hyperoxia and hypoxia
复制标题
DOI：
10.1152/ajplung.2001.281.4.l1021
复制
发表时间：
2001-10-01
影响因子：
4.9
通讯作者：
White, CW
中科院分区：
文献类型：
--
作者：
Allen, CB;Schneider, BK;White, CW
将培养细胞暴露于不断变化的气体环境中用作了解这种暴露对体内肺细胞的即时和长期影响的模型。我们用聚苯乙烯组织培养瓶和培养板进行了实验，以确定在体外条件下发生的氧浓度变化的时间过程。仅需几分钟，环境室中的氧气浓度就可达到与冲洗气体的平衡。然而，组织培养瓶和培养板中的培养基中的氧含量需要>3小时才能达到平衡。氧气在水溶液中的低溶解度和氧气通过培养基上方的气体边界层的有限扩散是氧气快速转运到培养基中的主要障碍。在培养基中获得所需Po-2的延迟限制了细胞事件与那些细胞所暴露的氧浓度的相关性的任何评估的时间精度。
Exposure of cultured cells to changing gaseous environments is used as a model for understanding both the immediate and long-term effects of such exposures on lung cells in vivo. We conducted experiments with polystyrene tissue culture flasks and plates to determine the time course of changes in oxygen concentration occurring under in vitro conditions. Only a few minutes were required for the concentration of oxygen in the environmental chamber to reach equilibrium with that of the flushing gas. However, >3 h were required for the oxygen content in the medium in the tissue culture flasks and plates to achieve equilibrium. The low solubility of oxygen in aqueous solutions and the limited diffusion of oxygen through a boundary layer of gas above the medium are the major barriers to rapid oxygen transport into the culture medium. The delay in achieving the desired Po-2 within the culture medium limits the temporal precision of any assessment of the correlation of cellular events with the concentration of oxygen to which those cells are exposed.