Luteinizing hormone-releasing hormone-induced luteinizing hormone secretion in vitro: cyclic changes in responsiveness and self-priming.
Luteinizing hormone-releasing hormone-induced luteinizing hormone secretion in vitro: cyclic changes in responsiveness and self-priming.
复制标题
黄体生成素释放激素诱导的体外黄体生成素分泌：反应性和自启动的周期性变化。
作者：
D. W. Waring;J. Turgeon
在 4 天动情周期的每个阶段从雌性大鼠中取出垂体前叶，分成四份，在零时间置于腔室中，并以 1.0 ml/min 灌注 360 分钟。在 120 分钟和 240 分钟时，给予 10 分钟的 LHRH (100 ng/ml) 脉冲。收集 10 或 2 分钟的流出液部分用于 LH 分析。测定了 LH 分泌总量和响应 LHRH 脉冲而达到的最大 LH 分泌率。 LHRH 第一次脉冲的 LH 分泌反应（或垂体反应性）在动情第 1 天和第 2 天最低，在发情前期最高，发情期处于中间状态。与发情前期早上取出的垂体相比，在 LH 激增开始前的发情前期下午取出的灌注垂体中，LH 对 LHRH 的反应明显更大。对 LHRH 第二次脉冲的 LH 分泌反应增强，即自启动效应，仅出现在动情第二天和发情前期。结果表明，循环...
Anterior pituitaries were removed from female rats at each stage of 4-day estrous cycles, quartered, placed in a chamber at time zero, and superfused at 1.0 ml/min for 360 min. At 120 and again at 240 min, a 10-min pulse of LHRH (100 ng/ml) was administered. Ten- or 2-min fractions of effluent fluid were collected for LH analysis. Both the total amount of LH secreted and the maximum LH secretory rate attained in response to pulses of LHRH were determined. The LH secretory response to the first pulse of LHRH, or pituitary responsiveness, was lowest on diestrous days 1 and 2 and maximal on proestrus, with estrus being intermediate. The LH response to LHRH was significantly greater in superfused pituitaries removed on the afternoon of proestrus before the onset of the LH surge compared to pituitaries taken on the morning of proestrus. An augmented LH secretory response to the second pulse of LHRH, the self-priming effect, was present on diestrous day 2 and proestrus only. The results demonstrate that the cyc...