An Assessment of the Effects of Azodicarbonamide-containing Diet on Neurobehaviour, Brain Antioxidant Status and Membrane Lipid Peroxidation Status in Rats
An Assessment of the Effects of Azodicarbonamide-containing Diet on Neurobehaviour, Brain Antioxidant Status and Membrane Lipid Peroxidation Status in Rats
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DOI：
10.2174/1871524919666191104154009
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发表时间：
2020-04-01
影响因子：
--
通讯作者：
Onaolapo, Olakunle J.
中科院分区：
文献类型：
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作者：
Olofinnade, Anthony T.;Adeyeba, Adegboyega;Onaolapo, Olakunle J.
背景：偶氮二甲酰胺是尼日利亚等国家面包制作过程中使用的面团增强剂。虽然有人认为它是呼吸系统的致敏剂，但缺乏关于其对中枢神经系统影响的信息。目的：本研究评估偶氮二甲酰胺对大鼠中枢神经系统（ADA）的影响。目的：探讨含ADA饮食对大鼠神经行为、脑抗氧化状态和选定脑区神经形态的影响。方法：将40只成年大鼠随机分为4组，每组10只，分别给予标准饮食或含1%、2%和4%ADA的饮食。给大鼠喂食标准饮食或含 ADA 的饮食 28 天。进行每周体重评估和每日食物摄入量估计。第 29 天进行行为测试（在开放场地、Y 型迷宫、径向臂迷宫和高架十字迷宫 (EPM)）。最后一次行为测试后 24 小时，对动物实施安乐死，解剖全脑、称重并均质化以评估脂质过氧化和抗氧化状态；结果：食用含 ADA 的饮食与体重增加/食物摄入量的显着减少以及水平运动和饲养行为的显着抑制相关；然而，梳理毛发的活动显着增加。此外，EPM 中的开臂时间显着减少，Y 迷宫交替显着增加（在 ADA 浓度最低时）。含 ADA 的饮食与大脑氧化状态或神经形态学的显着变化无关。结论：研究表明，含 ADA 的饮食可能会改变大鼠的神经行为；这与大脑氧化应激或神经组织形态学改变的证据无关。
Background: Azodicarbonamide is a dough-enhancer used in the process of bread-making in countries like Nigeria. While there have been suggestions that it is a sensitizer of the respiratory system, there is a dearth of information on its effects on the central nervous system. Aim: This study assessed the effects of azodicarbonamide on the central nervous system (ADA) in rats.Objective: The effects of ADA-containing diet on neurobehaviour, brain antioxidant status, and neuromorphology of selected brain regions in rats were examined.Methods: Forty adult rats were randomly-assigned into four groups of ten rats each, and were given standard diet or diet containing ADA at 1, 2 and 4% respectively. Rats were fed a standard diet or ADA-containing diet for a period of 28 days. Weekly body weight assessment and daily estimation of food intake were done. Behavioural tests {in the Open field, Y-maze, radial-arm maze, and Elevated Plus Maze (EPM)} were conducted on day 29. Twenty-four hours after the last behavioural test, animals were euthanised, whole brains were dissected, weighed, and either homogenised for assessment of lipid peroxidation and antioxidant status; or sectioned and processed for general histology.Results: Consumption of ADA-containing diet was associated with a significant decrease in weight gain/food intake, and significant suppression of horizontal locomotion and rearing behaviours; however, grooming activity increased significantly. Also, there was a significant reduction of open-arm time in the EPM and a significant increase in Y-maze alternation (at the lowest concentration of ADA). ADA-containing diet was not associated with significant changes in brain oxidative status or neuromorphology.Conclusion: The study showed that while ADA-containing diet may alter neurobehaviour in rats; this was not associated with evidence of brain oxidative stress or neuro-histomorphological alterations.