Metabolic Fingerprinting on Synthetic Alloys for Medulloblastoma Diagnosis and Radiotherapy Evaluation
Metabolic Fingerprinting on Synthetic Alloys for Medulloblastoma Diagnosis and Radiotherapy Evaluation
复制标题
用于髓母细胞瘤诊断和放射治疗评估的合成合金的代谢指纹图谱
DOI：
10.1002/adma.202000906
复制
发表时间：
2020-04-27
影响因子：
29.4
通讯作者：
Qian, Kun
中科院分区：
文献类型：
--
作者：
Cao, Jing;Shi, Xuejiao;Qian, Kun
诊断是癌症筛查和治疗的关键。作为精密医学中的新兴工具，代谢分析检测途径的终产物，因此比蛋白质组学/遗传分析更远。然而，由于样本的复杂性和患者样本中代谢物的丰富性，代谢分析在临床诊断中远非理想。另一个挑战是实时和准确地跟踪治疗效果，例如，放疗在这里，钯-金合成合金的报告为基础的质谱代谢指纹和分析，对髓母细胞瘤的诊断和放射治疗的评价。设计了一种核-壳结构，利用磁性核粒子在表面负载钯金合金。优化的合成合金增强了激光解吸/电离效率，并在几秒钟内直接检测100 nL的生物流体。通过代谢指纹的机器学习，髓母细胞瘤患者与健康对照者的平均诊断灵敏度为94.0%，特异性为85.7%，准确性为89.9%。此外，监测患者的放射治疗过程，并鉴定具有逐渐变化的血清代谢物生物标志物的初步组。这项工作将导致应用驱动的新材料的开发与定制的结构设计和建立新的协议，在不久的将来精确医学。
Diagnostics is the key in screening and treatment of cancer. As an emerging tool in precision medicine, metabolic analysis detects end products of pathways, and thus is more distal than proteomic/genetic analysis. However, metabolic analysis is far from ideal in clinical diagnosis due to the sample complexity and metabolite abundance in patient specimens. A further challenge is real-time and accurate tracking of treatment effect, e.g., radiotherapy. Here, Pd-Au synthetic alloys are reported for mass-spectrometry-based metabolic fingerprinting and analysis, toward medulloblastoma diagnosis and radiotherapy evaluation. A core-shell structure is designed using magnetic core particles to support Pd-Au alloys on the surface. Optimized synthetic alloys enhance the laser desorption/ionization efficacy and achieve direct detection of 100 nL of biofluids in seconds. Medulloblastoma patients are differentiated from healthy controls with average diagnostic sensitivity of 94.0%, specificity of 85.7%, and accuracy of 89.9%, by machine learning of metabolic fingerprinting. Furthermore, the radiotherapy process of patients is monitored and a preliminary panel of serum metabolite biomarkers is identified with gradual changes. This work will lead to the application-driven development of novel materials with tailored structural design and establishment of new protocols for precision medicine in near future.