Effect of acetylsalicylic acid on glutathione consumption and hexose monophosphate shunt during arachidonic acid induced stimulation of human blood platelets.
Effect of acetylsalicylic acid on glutathione consumption and hexose monophosphate shunt during arachidonic acid induced stimulation of human blood platelets.
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乙酰水杨酸对花生四烯酸诱导刺激人血小板期间谷胱甘肽消耗和单磷酸己糖分流的影响。
DOI：
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发表时间：
1984
期刊：
影响因子：
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通讯作者：
U. Till
中科院分区：
文献类型：
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作者：
W. Lösche;G. Pescarmona;J. Hofmann;A. Bosìa;P. Arese;K. Thielmann;U. Till
外源性花生四烯酸引起的人血小板聚集伴随着通过磷酸己糖分流的净流量的有力增加和还原型谷胱甘肽水平的降低。当血小板环氧合酶被乙酰水杨酸抑制时，用其他谷胱甘肽氧化剂如二酰胺或叔丁基过氧化氢进行的研究表明，乙酰水杨酸既不影响谷胱甘肽提供酶的活性，也不影响谷胱甘肽过氧化物酶的活性，在花生四烯酸代谢中催化谷胱甘肽消耗反应。与文献数据一起，结果指出血小板中环氧合酶和脂氧合酶途径中辅酶的消耗减少。
Aggregation of human blood platelets by exogenous arachidonic acid is accompanied by a powerful increase in the net flux through the hexose monophosphate shunt and a decrease of the level of reduced glutathione. When platelet cyclooxygenase is inhibited by acetylsalicylic acid diminution by about 60% of arachidonic acid induced flux through the hexose monophosphate shunt as well as lower initial decrease of the glutathione level are found. Investigations with other glutathione oxidizing agents as diamide or tertiary butyl hydroperoxide reveal that acetylsalicylic acid influences neither the activities of glutathione providing enzymes nor that of glutathione peroxidase which catalyzes glutathione consuming reactions in the arachidonic acid metabolism. Together with literature data the results point to a consumption of reduced coenzymes in both cyclooxygenase and lipoxygenase pathways in platelets.