IDENTIFICATION OF QUANTITATIVE TRAIT LOCI CONTROLLING DAYS TO FLOWERING AND PLANT HEIGHT IN 2 NEAR-ISOGENIC LINES OF MAIZE
IDENTIFICATION OF QUANTITATIVE TRAIT LOCI CONTROLLING DAYS TO FLOWERING AND PLANT HEIGHT IN 2 NEAR-ISOGENIC LINES OF MAIZE
复制标题
DOI：
10.2135/cropsci1993.0011183x003300060020x
复制
发表时间：
1993-11-01
期刊：
影响因子：
2.3
通讯作者：
STUBER, CW
中科院分区：
文献类型：
--
作者：
KOESTER, RP;SISCO, PH;STUBER, CW
从种植到开花的天数是玉米（Zea mays L.）育种家认为，它在选择合适的杂交亲本方面很重要，在利用不适应的种质方面也很重要。利用分子标记技术，在两个近等基因系（NILs）中检测到了控制开花天数和两个相关性状（株高和总叶片数）的数量性状基因座（QTL）。NC 264和B73 G分别是SC 76和B73的较短、较早的版本，通过渗入加斯佩弗林特并通过重复回交选择早花而开发。用限制性片段长度多态性（RFLP）筛选近等基因系的渐渗染色体区域。在NC 264和B73 G中分别鉴定出7个基因渗入区域，在两个近等基因系中均保持有特定的8号染色体区域。在分离的F-2群体和由原始自交系与近等基因系之间的杂交开发的F-3家系中测试渐渗区域对开花日期和株高的影响。NC 264 x SC 76 F-2群体在长日照和短日照两种环境中进行了测试。通过对F-2和F-3家系的RFLP分析，在第1、8和10号染色体上发现了影响开花天数和株高的主效QTL。控制总叶数的主效QTL位于第1和第8染色体上。单因素方差分析技术，采用所有成对标记性状协会。加性基因作用在所有位点占主导地位。最显着的影响是恒定的跨环境，代，和人口，除了该地区的8号染色体，这是不显着的短日照环境。因此，8号染色体上的成熟度QTL可能代表光周期反应元件。选择性测定基因型，只使用顶部和底部的10%的表型极端，以确定QTL是有效的分析整个人口检测最显着的标记性状协会。
The number of days from planting to flowering is a trait of interest to maize (Zea mays L.) breeders for its importance in selecting appropriate hybrid parents, and for its role in the utilization of unadapted germplasm. Using molecular marker technology, we were able to identify quantitative trait loci (QTLs) controlling days to flowering and two correlated traits, plant height and total leaf number, in two near isogenic lines (NILs). NC264 and B73G are shorter, earlier versions of SC76 and B73 respectively, developed by introgressing Gaspe Flint and selecting for early flowering through repeated backcrosses. The NILs were screened for introgressed chromosomal regions with restriction fragment length polymorphisms (RFLPs). Seven introgressed regions were identified in NC264 and two in B73G, with a specific chromosome 8 region maintained in both NILs. The introgressed regions were tested for their effect on flowering date and plant height in segregating F-2 populations and F-3 families developed from crosses between the original inbred and the NIL. The NC264 x SC76 F-2 population was tested in both long and short-day photoperiod environments. The RFLP analysis of the F-2 individuals and F-3 families identified major QTLs for days to flowering and plant height on chromosomes 1, 8, and 10. Major QTLs for total leaf number were found on chromosomes 1 and 8. Single-factor analysis of variance techniques were employed for all pairwise marker trait associations. Additive gene action predominated at all loci. The most significant effects were constant across environments, generations, and populations except for the region on chromosome 8, which was not significant in the short-day photoperiod environment. Thus, the maturity QTL on chromosome 8 may represent a photoperiod response element. Selective determination of genotype using only the top and bottom 10% of the phenotypic extremes to identify QTLs was as effective as analysis of the entire population for detecting the most significant marker trait associations.