Fluorescent Cell-Conjugation by a Multifunctional Polymer: A New Application of the Hantzsch Reaction
Fluorescent Cell-Conjugation by a Multifunctional Polymer: A New Application of the Hantzsch Reaction
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多功能聚合物的荧光细胞缀合：Hantzsch 反应的新应用
DOI：
10.1021/acsmacrolett.7b00220
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发表时间：
2017
影响因子：
5.8
通讯作者：
Tao Lei
中科院分区：
文献类型：
--
作者：
Sun Qiang;Liu Guoqiang;Wu Haibo;Xue Haodong;Zhao Yuan;Wang Zilin;Wei Yen;Wang Zhiming;Tao Lei
多组分反应（mcr）可以形成独特的结构和有趣的功能，因此，可以简单地利用mcr作为偶联工具，同时连接和生成不同的官能团来制备多功能聚合物。为了验证这一概念，将Hantzsch反应与可逆加成-破碎链转移（RAFT）聚合相结合，通过一锅法制备了一种新型的苯基硼酸荧光聚合物。Hantzsch-RAFT系统已经被发现可以平滑地实现预先设计的多功能聚合物，它可以通过苯硼酸和细胞膜上的糖蛋白之间的相互作用用于细胞偶联。由于聚合物链上荧光的Hantzsch部分，可以直接观察到共轭细胞，展示了老Hantzsch反应（>130年）在有机化学以外的新应用。同时，结合后的细胞在凝血蛋白（凝集素）存在下仍保持良好的分散性，这表明多功能聚合物可能是一种用于细胞分离的抗凝剂。我们相信，本研究为mcr在跨学科领域的新应用开辟了一条新途径，并可能促进基于不同mcr的其他多功能聚合物的发展。
Multicomponent reactions (MCRs) can form unique structures with interesting functions, therefore, multifunctional polymers might be simply prepared using MCRs as coupling tools to simultaneously link and generate different functional groups. To verify this concept, a new fluorescent polymer containing phenylboronic acid has been facilely prepared via a one pot method by combining the Hantzsch reaction with reversible addition–fragmentation chain transfer (RAFT) polymerization. The Hantzsch-RAFT system has been found robust to smoothly achieve predesigned multifunctional polymer, which can be used for cell conjugation through the interaction between phenylboronic acid and glycoprotein on cell membrane. The conjugated cells could be directly observed due to the fluorescent Hantzsch moiety in the polymer chain, demonstrating a new application of the old Hantzsch reaction (>130 years) outside organic chemistry. Meanwhile, the conjugated cells remained excellent dispersity in the presence of coagulation protein (lectin), implying that multifunctional polymer a possible anticoagulant for cell separation. We believe that the current research paves a new way to exploit new applications of MCRs in interdisciplinary fields and might prompt the development of other multifunctional polymers based on different MCRs.