Efficient 2,3-butanediol production from whey powder using metabolically engineered Klebsiella oxytoca
Efficient 2,3-butanediol production from whey powder using metabolically engineered Klebsiella oxytoca
复制标题
利用代谢工程产酸克雷伯菌从乳清粉中高效生产 2,3-丁二醇
DOI：
10.1186/s12934-020-01420-2
复制
发表时间：
2020-08-10
影响因子：
6.4
通讯作者：
Ma, Cuiqing
中科院分区：
文献类型：
--
作者：
Meng, Wensi;Zhang, Yongjia;Ma, Cuiqing
背景
乳清是乳制品工业产生的主要污染物。为了减少乳清工业排放造成的环境污染，迫切需要探索乳清利用的新前景。在这里，我们研究了使用乳清粉生产 2,3-丁二醇 (BDO)（一种重要的平台化学品）的可能性。
结果
选择克雷伯氏菌菌株 PDL-0 是因为它能够从乳糖（乳清中的主要可发酵糖）中高效生产 BDO。删除与乙酸、琥珀酸、乳酸和甲酸副产物产生相关的基因pox、pta、frdA、ldhD和pflB后，构建了重组菌株K. oxytoca PDL-K5。使用 K. oxytoca PDL-K5 的补料分批发酵产生了 74.9 g/L BDO，生产率为 2.27 g/L/h，乳糖产量为 0.43 g/g。此外，当使用乳清粉作为底物时，24小时内产生了65.5 g/L BDO，生产率为2.73 g/L/h，收率为0.44 g/g。
结论
这项研究证明了 K. oxytoca PDL-0 从乳清中生产 BDO 的效率。由于其非致病性和有效的乳糖利用，K. oxytoca PDL-0 也可用于以乳清为底物生产其他重要化学品。
Background
Whey is a major pollutant generated by the dairy industry. To decrease environmental pollution caused by the industrial release of whey, new prospects for its utilization need to be urgently explored. Here, we investigated the possibility of using whey powder to produce 2,3-butanediol (BDO), an important platform chemical.
Results
Klebsiella oxytoca strain PDL-0 was selected because of its ability to efficiently produce BDO from lactose, the major fermentable sugar in whey. After deleting genes pox, pta, frdA, ldhD, and pflB responding for the production of by-products acetate, succinate, lactate, and formate, a recombinant strain K. oxytoca PDL-K5 was constructed. Fed-batch fermentation using K. oxytoca PDL-K5 produced 74.9 g/L BDO with a productivity of 2.27 g/L/h and a yield of 0.43 g/g from lactose. In addition, when whey powder was used as the substrate, 65.5 g/L BDO was produced within 24 h with a productivity of 2.73 g/L/h and a yield of 0.44 g/g.
Conclusion
This study demonstrated the efficiency of K. oxytoca PDL-0 for BDO production from whey. Due to its non-pathogenicity and efficient lactose utilization, K. oxytoca PDL-0 might also be used in the production of other important chemicals using whey as the substrate.