Bdellovibrio bacteriovorus directly attacks Pseudomonas aeruginosa and Staphylococcus aureus Cystic fibrosis isolates
Bdellovibrio bacteriovorus directly attacks Pseudomonas aeruginosa and Staphylococcus aureus Cystic fibrosis isolates
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DOI：
10.3389/fmicb.2014.00280
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发表时间：
2014-06-05
影响因子：
5.2
通讯作者：
Schippa, Serena
中科院分区：
文献类型：
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作者：
Iebba, Valerio;Totino, Valentina;Schippa, Serena
噬菌蛭弧菌是一种在环境和人类肠道内发现的捕食性细菌，能够攻击革兰氏阴性猎物。囊性纤维化（CF）是一种遗传性疾病，通常表现为铜绿假单胞菌或金黄色葡萄球菌生物膜在肺部定植。在这里，我们通过以下方法研究了B. bactenovorus对这两种致病菌的捕食行为：（1）肉汤培养； (2)“静态”生物膜； (3)场发射扫描电子显微镜(FESEM)； (4)“流动”生物膜； (5)酶谱技术。我们获得了食菌芽胞杆菌在革兰氏阳性猎物中存活的第一个证据，揭示了与金黄色葡萄球菌的直接细胞间接触以及用 FESEM 成像的新的“表生”觅食策略。 HD100对金黄色葡萄球菌细胞的平均附着时间为185秒，而“静态”和“流动”金黄色葡萄球菌生物膜分别减少了74％（24小时）和46％（20小时）。此外，酶谱显示食菌芽孢杆菌裂解物对铜绿假单胞菌和金黄色葡萄球菌具有不同的溶菌活性。针对革兰氏阴性（周质）和革兰氏阳性（表生）猎物的双重觅食系统可能建议使用食菌芽孢杆菌作为 CF 中的“活抗生素”，即使需要进一步研究来模拟其体内捕食行为。
Bdellovibrio bacteriovorus is a predator bacterial species found in the environment and within the human gut, able to attack Gram-negative prey. Cystic fibrosis (CF) is a genetic disease which usually presents lung colonization by Pseudomonas aeruginosa or Staphylococcus aureus biofilms. Here, we investigated the predatory behavior of B. bactenovorus against these two pathogenic species with: (1) broth culture; (2) "static" biofilms; (3) field emission scanning electron microscope (FESEM); (4) "flow" biofilms; (5) zymographic technique. We had the first evidence of B. bacteriovorus survival with a Gram-positive prey, revealing a direct cell-to-cell contact with S. aureus and a new "epibiotic" foraging strategy imaged with FESEM. Mean attaching time of HD100 to S. aureus cells was 185s, while "static" and "flow" S. aureus biofilms were reduced by 74 (at 24h) and 46% (at 20h), respectively. Furthermore, zymograms showed a differential bacteriolytic activity exerted by the B. bacteriovorus lysates on P aeruginosa and S. aureus. The dual foraging system against Gram-negative (periplasmic) and Gram-positive (epibiotic) prey could suggest the use of B. bacteriovorus as a "living antibiotic" in CF, even if further studies are required to simulate its in vivo predatory behavior.