Comparison of the induction and disappearance of DNA double strand breaks and γ-H2AX foci after irradiation of chromosomes in G1-phase or in condensed metaphase cells
Comparison of the induction and disappearance of DNA double strand breaks and γ-H2AX foci after irradiation of chromosomes in G1-phase or in condensed metaphase cells
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DOI：
10.1016/j.mrfmmm.2007.11.006
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发表时间：
2008-03-01
影响因子：
2.3
通讯作者：
Bedford, Joel S.
中科院分区：
文献类型：
--
作者：
Kato, Takamitsu A.;Okayasu, Ryuichi;Bedford, Joel S.
用γ - h2ax法和凝胶电泳法比较G1和有丝分裂细胞辐照后DNA双链断裂（DSBs）的诱导和消失情况。这是为了确定细胞周期相关的染色质结构变化是否可能影响γ -H2AX测定，这取决于dsb周围H2AX组蛋白变体的广泛磷酸化和去磷酸化。有丝分裂后γ - h2ax病灶的消失速度比G1细胞慢得多。另一方面，凝胶电泳分析没有发现差异：我们的数据可能表明，在辐照中期细胞或浓缩染色质中捕获γ - h2ax的去磷酸化酶的可及性有限。(c) 2007 Elsevier B.V.版权所有
The induction and disappearance of DNA double strand breaks (DSBs) after irradiation of G1 and mitotic cells were compared with the gamma-H2AX foci assay and a gel electrophoresis assay. This is to determine whether cell cycle related changes in chromatin structure might influence the gamma-H2AX assay which depends on extensive phosphorylation and dephosphorylation of the H2AX histone variant surrounding DSBs. The disappearance of gamma-H2AX foci after irradiation was much slower for mitotic than for G1 cells. On the other hand, no difference was seen for the gel electrophoresis assay: Our data may suggest the limited accessibility of dephosphorylation enzyme in irradiated metaphase cells or trapped gamma-H2AX in condensed chromatin. (c) 2007 Elsevier B.V. All rights reserved.