Metaproteomics of aquatic microbial communities in a deep and stratified estuary
Metaproteomics of aquatic microbial communities in a deep and stratified estuary
复制标题
DOI：
10.1002/pmic.201500079
复制
发表时间：
2015-10-01
期刊：
影响因子：
3.4
通讯作者：
Walsh, David A.
中科院分区：
文献类型：
--
作者：
Colatriano, David;Ramachandran, Arthi;Walsh, David A.
在这里，我们利用元蛋白质组学的力量来评估位于加拿大东部的深层和广阔的下圣劳伦斯河口的分层水生微生物群落的代谢多样性和功能。通过分层水柱的微生物群落的垂直剖面揭示了代谢生活方式和碳和氮处理途径的差异。在生产性地表沃茨，我们确定了异养种群参与处理的高分子量和低分子量的有机物从陆地（如纤维素和木糖）和海洋（如有机相容的渗透剂）来源。在生产力较低的深水沃茨，化学合成生产耦合硝化的MG-I Thaumarchaeota和Nitrospina似乎是一个占主导地位的代谢策略。类似于其他研究的沿海海洋，我们确定了甲醇氧化蛋白起源于共同的OM 43海洋分支。然而，我们还确定了一个新的谱系甲醇氧化剂，特别是在颗粒丰富的底部（即nepheloid）层。分配给未培养的MG-II Euryarchaeota的膜转运蛋白也被特异性地检测到在nepheloid层。总的来说，这些结果揭示了强大的垂直结构的微生物类群和代谢活动，以及特定的nepheloid类群，可能有助于显着沿海海洋养分循环的存在。
Here we harnessed the power of metaproteomics to assess the metabolic diversity and function of stratified aquatic microbial communities in the deep and expansive Lower St. Lawrence Estuary, located in eastern Canada. Vertical profiling of the microbial communities through the stratified water column revealed differences in metabolic lifestyles and in carbon and nitrogen processing pathways. In productive surface waters, we identified heterotrophic populations involved in the processing of high and low molecular weight organic matter from both terrestrial (e.g. cellulose and xylose) and marine (e.g. organic compatible osmolytes) sources. In the less productive deep waters, chemosynthetic production coupled to nitrification by MG-I Thaumarchaeota and Nitrospina appeared to be a dominant metabolic strategy. Similar to other studies of the coastal ocean, we identified methanol oxidation proteins originating from the common OM43 marine clade. However, we also identified a novel lineage of methanol-oxidizers specifically in the particle-rich bottom (i.e. nepheloid) layer. Membrane transport proteins assigned to the uncultivated MG-II Euryarchaeota were also specifically detected in the nepheloid layer. In total, these results revealed strong vertical structure of microbial taxa and metabolic activities, as well as the presence of specific nepheloid taxa that may contribute significantly to coastal ocean nutrient cycling.