An etched porous interface for on-line capillary electrophoresis-based two-dimensional separation system
An etched porous interface for on-line capillary electrophoresis-based two-dimensional separation system
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DOI：
10.1021/ac0493267
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发表时间：
2004-11-01
影响因子：
7.4
通讯作者：
Zhang, YK
中科院分区：
文献类型：
--
作者：
Liu, HC;Zhang, LH;Zhang, YK
描述了用于毛细管等电聚焦 (CIEF) 与毛细管区带电泳 (CZE) 在线耦合的柱上蚀刻熔融石英多孔连接的构建和评估。其中两个分离柱通过沿毛细管蚀刻的类似于 4 至 5 毫米长的多孔连接处集成在单件熔融石英毛细管上。去除聚酰亚胺涂层后，用 HF 蚀刻毛细管壁的一小部分即可轻松制备结。蚀刻部分变成多孔玻璃膜，当在分离毛细管上施加高电压时，仅允许与背景电解质相关的小离子通过。与以前的设计（其中界面通常是通过使毛细管破裂，然后用一段微透析中空纤维膜连接两个毛细管形成）相比，这种新型多孔连接界面的主要优点是无死体积、简单且坚固，特别适合基于在线耦合毛细管电泳的多维分离系统。通过分析蛋白质混合物来评估由这种蚀刻多孔连接构建的 2D CIEF-CZE 系统的性能。
The construction and evaluation of an on-column etched fused-silica porous junction for on-line coupling of capillary isoelectric focusing (CIEF) with capillary zone electrophoresis (CZE) are described. Where two separation columns were integrated on a single piece of fused-silica capillary through the etched similar to4 to 5-mm length porous junction along the capillary. The junction is easily prepared by etching a short section of the capillary wall with HF after removing the polyimide coating. The etched section becomes a porous glass membrane that allows only small ions related to the background electrolyte to pass through when high voltage is applied across the separation capillary. The primary advantages of this novel porous junction interface over previous designs (in which the interface is usually formed by fracturing the capillary followed by connecting the two capillaries with a section of microdialysis hollow fiber membrane) are no dead volume, simplicity, and ruggedness, which is particularly well suited for an on-line coupling capillary electrophoresis-based multiple dimensional separation system. The performance of the 2D CIEF-CZE system constructed by such an etched porous junction was evaluated by the analyses of protein mixtures.