AN RFLP LINKAGE MAP FOR LOBLOLLY-PINE BASED ON A 3-GENERATION OUTBRED PEDIGREE
AN RFLP LINKAGE MAP FOR LOBLOLLY-PINE BASED ON A 3-GENERATION OUTBRED PEDIGREE
复制标题
DOI：
10.1007/bf00223631
复制
发表时间：
1994-06-01
影响因子：
5.4
通讯作者：
NEALE, DB
中科院分区：
文献类型：
--
作者：
DEVEY, ME;FIDDLER, TA;NEALE, DB
火炬松的遗传连锁图谱是利用一个由四个祖父母、两个亲本和95个F-2后代组成的三代远交家系的分离数据构建的。该图谱主要基于cDNA探针检测到的限制性片段长度多态(RFLP)基因座。65个cDNA和3个基因组DNA探针共检测到90个RFLP基因座。对6个同工酶基因座进行了遗传多样性分析。四分之一(24%)的cDNA探针检测到一个以上的分离位点，这表明多基因家族在松树中是常见的。在祖父母之间的一个分离座位上观察到多达6个等位基因，后代每个座位分离3到4个等位基因并不少见。多点连锁分析将73个RFLP和2个同工酶座位归入20个连锁群，其余17个RFLP和4个同工酶座位未连锁。定位的RFLP探针为火炬松的遗传分析提供了一组新的共显性标记。
A genetic linkage map for loblolly pine (Pinus taeda L.) was constructed using segregation data from a three-generation outbred pedigree consisting of four grandparents, two parents, and 95 F-2 progeny. The map was based predominantly on restriction fragment length polymorphism (RFLP) loci detected by cDNA probes. Sixty-five cDNA and three genomic DNA probes revealed 90 RFLP loci. Six polymorphic isozyme loci were also scored. One-fourth (24%) of the cDNA probes detected more than 1 segregating locus, an indication that multigene families are common in pines. As many as six alleles were observed at a single segregating locus among grandparents and it was not unusual for the progeny to segregate for three or four alleles per locus. Multipoint linkage analysis placed 73 RFLP and 2 isozyme loci into 20 linkage groups; the remaining 17 RFLP and 4 isozyme loci were unlinked. The mapped RFLP probes provide a new set of codominant markers for genetic analyses in loblolly pine.