A mutation in the 3-phosphoglycerate kinase gene allows anaerobic growth of Bacillus subtilis in the absence of ResE kinase.
A mutation in the 3-phosphoglycerate kinase gene allows anaerobic growth of Bacillus subtilis in the absence of ResE kinase.
复制标题
3-磷酸甘油酸激酶基因的突变允许枯草芽孢杆菌在没有 ResE 激酶的情况下厌氧生长。
DOI：
10.1128/jb.181.22.7087-7097.1999
复制
发表时间：
1999
影响因子：
3.2
通讯作者：
Zuber,P
中科院分区：
文献类型：
--
作者：
Nakano,MM;Zhu,Y;Haga,K;Yoshikawa,H;Sonenshein,AL;Zuber,P
TheBacillus subtilisResD-ResE two-component signal transduction system is essential for aerobic and anaerobic respiration. A spontaneous suppressor mutant that expresses ResD-controlled genes and grows anaerobically in the absence of the ResE histidine kinase was isolated. In addition, aerobic expression of ResD-controlled genes in the suppressed strain was constitutive and occurred at a much higher level than that observed in the wild-type strain. The suppressing mutation, which mapped topgk, the gene encoding 3-phosphoglycerate kinase, failed to suppress aresDmutation, suggesting that the suppressing mutation creates a pathway for phosphorylation of the response regulator, ResD, which is independent of the cognate sensor kinase, ResE. Thepgk-1mutant exhibited very low but measurable 3-phosphoglycerate kinase activity compared to the wild-type strain. The results suggest that accumulation of a glycolytic intermediate, probably 1,3-diphosphoglycerate, is responsible for the observed effect of thepgk-1mutation on anaerobiosis ofresEmutant cells.
