High histone deacetylase 7 (HDAC7) expression is significantly associated with adenocarcinomas of the pancreas
High histone deacetylase 7 (HDAC7) expression is significantly associated with adenocarcinomas of the pancreas
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DOI：
10.1245/s10434-008-9940-z
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发表时间：
2008-08-01
影响因子：
3.7
通讯作者：
Ouaissi, Ali
中科院分区：
文献类型：
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作者：
Ouaissi, Mehdi;Sielezneff, Igor;Ouaissi, Ali
背景：HDACs基因表达的改变已经在许多人类癌症中报道。没有关于HDAC在胰腺癌肿瘤中的状态的信息。本研究的目的是评估I类成员的表达水平，（HDAC 1、2、3），II类（HDAC 4、5、6和7）和III类方法：从11例胰腺癌（PA）中分离总RNA：0期（n = 1）、IB期（n = 1）、IIB期（n = 6）、III期（n = 1）、IV期（n = 2）、一个浆液性囊腺瘤（SC）、一个胰腺导管内乳头状粘液性肿瘤（IMPN）、一个并发慢性胰腺炎（CP）和在供体肝移植期间获得的正常胰腺（NP）。此外，还包括6个其他对照胰腺。使用定量实时聚合酶链反应（qPCR）进行HDAC基因表达。蛋白质表达水平进行了分析，Western blot和免疫组化分析癌组织sect.Results的定位：值得注意的是，9的11 PA（约81%）显示HDAC7 mRNA水平显着增加。与PA样品相比，CP、SC和IMPN标本中HDAC7的信息减少。蛋白质印迹分析显示HDAC7蛋白在11个PA样品中的9个中表达增加，与qPCR数据一致。结论：胰腺癌组织中HDAC7基因表达有明显的改变。HDAC7表达增加可将PA与其他胰腺肿瘤区分开来。
Background: Alterations in HDACs gene expression have been reported in a number of human cancers. No information is available concerning the status of HDACs in pancreatic cancer tumors. The aim of the present study was to evaluate the expression levels of members of class I (HDAC1, 2,, 3), class II (HDAC4, 5, 6, and 7), and class III (SIRT1, 2, 3, 4, 5, and 6) in a set of surgically resected pancreatic tissues.Methods: Total RNA was isolated from 11 pancreatic adenocarcinomas (PA): stage 0 (n = 1), IB (n = 1), IIB (n = 6), III (n = 1), IV (n = 2), one serous cystadenoma (SC), one intraductal papillary mucinous tumor of the pancreas (IMPN), one complicating chronic pancreatitis (CP), and normal pancreas (NP) obtained during donor liver transplantation. Moreover, six other control pancreatic were included. HDACs gene expression was conducted using quantitative real-time polymerase chain reaction (qPCR). Protein expression levels were analyzed by Western blot and their localization by immunohistochemistry analyses of cancer tissues sections.Results: Remarkably, 9 of the 11 PA (approximately 81%) showed significant increase of HDAC7 mRNA levels. In contrast to PA samples, message for HDAC7 was reduced in CP, SC, and IMPN specimens. The Western blot analysis showed increased expression of HDAC7 protein in 9 out of 11 PA samples, in agreement with the qPCR data. Most of the PA tissue sections examined showed intense labeling in the cytoplasm when reacted against antibodies to HDAC7.Conclusion: The data showed alteration of HDACs gene expression in pancreatic cancer. Increased expression of HDAC7 discriminates PA from other pancreatic tumors.