Nobiletin Inhibits PDGF-BB-Induced Vascular Smooth Muscle Cell Proliferation and Migration and Attenuates Neointimal Hyperplasia in a Rat Carotid Artery Injury Model
Nobiletin Inhibits PDGF-BB-Induced Vascular Smooth Muscle Cell Proliferation and Migration and Attenuates Neointimal Hyperplasia in a Rat Carotid Artery Injury Model
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DOI：
10.1002/ddr.21230
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发表时间：
2014-12-01
影响因子：
3.8
通讯作者：
Li, Bin
中科院分区：
文献类型：
--
作者：
Guan, Siyu;Tang, Qizhu;Li, Bin
血管平滑肌细胞（VSMCs）的异常迁移和增殖在血管损伤后新生内膜增生的发展中起关键作用。川陈皮素是一种柑橘类生物黄酮，具有抗炎和抗氧化活性。本研究评估了川陈皮素是否能抑制血小板衍生生长因子（PDGF）-BB刺激的血管平滑肌细胞增殖和迁移，并减少大鼠颈动脉损伤模型中的新生内膜增生。从大鼠胸主动脉培养的血管平滑肌细胞在用20ng/ml PDGF - BB刺激之前用川陈皮素处理，并且大鼠接受颈动脉损伤。川陈皮素抑制PDGF - BB诱导的血管平滑肌细胞增殖和迁移，减弱活性氧（ROS）的产生，并降低PDGF - BB刺激的血管平滑肌细胞中ERK1/2的磷酸化以及核因子NF - κB p65的表达。川陈皮素减少了球囊损伤大鼠颈动脉的内膜面积以及新生内膜与中膜的比率。川陈皮素处理的大鼠血清中肿瘤坏死因子 - α和白细胞介素 - 6水平降低。这些结果表明川陈皮素可能是一种用于预防和治疗血管成形术后再狭窄的潜在保护剂。（C）2014威利期刊公司
The abnormal migration and proliferation of vascular smooth muscle cells (VSMCs) plays a pivotal role in the development of neointimal hyperplasia after vascular injury. Nobiletin, a citrus bioflavonoid, exhibits anti-inflammatory and anti-oxidative activities. The present study evalutaed whether nobiletin could inhibit platelet-derived growth factor (PDGF)-BB- stimulated VSMC proliferation and migration and decrease neointimal hyperplasia in a rat carotid artery injury model. Cultured VSMCs from rat thoracic aortas were treated with nobiletin before being stimulated with 20ng/ml PDGF-BB, and rats were subjected to carotid artery injury. Nobiletin inhibited PDGF-BB-induced VSMC proliferation and migration, attenuated reactive oxygen species (ROS) production and reduced phosphorylation of ERK1/2 and the expression of nuclear NF-B p65 in PDGF-BB-stimulated VSMCs. Nobiletin decreased the intima area and the ratio of neointima to media in balloon-injured rat carotid arteries. Serum levels of TNF- and IL-6 in nobiletin-treated rats were decreased. These results indicated that nobiletin could be a potential protective agent for the prevention and treatment of restenosis after angioplasty. (C) 2014 Wiley Periodicals, Inc.