A simple in vitro test to monitor trace metal toxicity in aqueous samples
A simple in vitro test to monitor trace metal toxicity in aqueous samples
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监测水样中痕量金属毒性的简单体外测试
DOI：
10.1080/09593339209385209
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发表时间：
1992
影响因子：
2.8
通讯作者：
P. Bernardi
中科院分区：
文献类型：
--
作者：
M. Bragadin;E. Argese;A. Nicolli;P. Bernardi
摘要：我们在简单的分光光度测定中使用氧化细胞色素 c 作为电子受体，研究了测试毒物对沿着线粒体外膜的鱼藤酮不敏感 NADH 细胞色素 b5 还原酶的电子传递的影响。我们证明，该反应对环境中存在的微量金属极其敏感，可以可靠地检测到低至 10 nM Hg2+ 的汞。该测定方法重复性好、简单、快速且廉价，与鱼类急性毒性测试相比具有优势，并且可用于对水域中此类污染物的存在进行常规监测。
Abstract We have studied the effect of test toxicants on electron transfer along the rotenone‐insensitive NADH‐cytochrome b5 reductase of the outer mitochondrial membrane, using oxidized cytochrome c as the electron acceptor in a simple spectrophotometric assay. We show that this reaction is extremely sensitive to trace metals of environmental concern, detecting reliably as little as 10 nM Hg2+. The assay is reproducible, simple, fast and inexpensive, compares favourably with fish acute toxicity tests, and may become useful in the routine monitoring of waters for the presence of this class of pollutants.