COMPARISON BETWEEN INVITRO RADIOSENSITIVITY AND INVIVO RADIORESPONSE IN MURINE TUMOR-CELL LINES .2. INVIVO RADIORESPONSE FOLLOWING FRACTIONATED TREATMENT AND INVITRO INVIVO CORRELATIONS
COMPARISON BETWEEN INVITRO RADIOSENSITIVITY AND INVIVO RADIORESPONSE IN MURINE TUMOR-CELL LINES .2. INVIVO RADIORESPONSE FOLLOWING FRACTIONATED TREATMENT AND INVITRO INVIVO CORRELATIONS
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DOI：
10.1016/0360-3016(90)90098-5
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发表时间：
1990-02-01
影响因子：
7
通讯作者：
HILL, RP
中科院分区：
文献类型：
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作者：
BRISTOW, RG;HILL, RP
人类肿瘤细胞系体外辐射存活曲线低剂量区域的存活率可能与类似组织病理学类型的肿瘤的预期临床放射治愈性相关。目前的研究使用一系列可移植的小鼠实体瘤检验了这一假设。通过生长延迟和肿瘤细胞存活测定来测量分次剂量（以 4 小时间隔给予 10 次 2 Gy）和单剂量（20 Gy）放射治疗后肿瘤的体内放射反应。肿瘤细胞存活的测量在放射治疗结束后立即或8小时开始。没有证据表明任何肿瘤体内潜在致命损伤的修复。将体内反应的测量结果与同时测量的相同肿瘤细胞系的体外辐射存活曲线参数进行比较。 10 次 2 Gy 照射后的肿瘤细胞存活率与 2 Gy 时体外测量的存活率相关性最好，尽管根据体外数据计算的两个参数也观察到良好的相关性； .alpha 的值。通过拟合线性二次 (LQ) 模型和平均灭活剂量 (MID)。还观察到 10 次 2 Gy 剂量后体内测量的特定生长延迟与这些体外参数之间的相关性。尽管观察到相关性，但在体内 10 次 2 Gy 剂量后测量的存活值与假设每次剂量效果相同的体外存活数据预测的理论值在数量上并不一致。这种差异表明其他因素也影响肿瘤对分段照射的总体反应。尽管如此，这些发现支持了这样的观点，即内在放射敏感性在确定肿瘤对分段照射的总体反应中起着重要作用，并为测试活检标本的体外放射敏感性作为临床放射治疗能力的预测测定的概念提供了强有力的支持。
Survival in the low-dose region of in vitro radiation survival curves for human tumor cell lines may be correlated with the expected clinical radiocurability of tumors of similar histopathological type. The present investigation examined this hypothesis using a series of transplantable murine solid tumors. The in vivo radioresponse of the tumors following fractionated dose (10 fractions of 2 Gy given at 4 hr intervals) and single dose (20 Gy) radiation treatment was measured by growth delay and tumor cell survival assays. The measurements of tumor cell survival were initiated either immediately or 8 hr after the end of radiation treatment. There was no evidence for repair of potentially lethal damage in vivo in any of the tumors. The measurements of in vivo response were compared to parameters of in vitro radiation survival curves for the same tumor cell lines, which were measured concurrently. The tumor cell survival following 10 fractions of 2 Gy correlated best with the measured in vitro survival at 2 Gy, although good correlations were also observed with two parameters calculated from the in vitro data; the value of .alpha. from fitting the linear-quadratic (LQ) model and the mean inactivation dose (MID). Correlations were also observed between specific growth delay measured in vivo following 10 fractions of 2 Gy and these in vitro parameters. Despite the correlations observed, the measured survival values following 10 fractions of 2 Gy in vivo did not agree quantitatively with the theoretical values predicted from the in vitro survival data assuming equal effect for each fraction. This discrepancy indicates that other factors also contribute to the overall response of a tumor to fractionated irradiation. Neverthless, these findings support the idea that intrinsic radiosensitivity plays a significant role in determining the overall response of a tumor to fractionated irradiation and provides strong support for the concept of testing the in vitro radiation sensitivity of biopsy specimens as a predictive assay of clinical radiocurability.