Antibacterial activity of chitosans and chitosan oligomers with different molecular weights
Antibacterial activity of chitosans and chitosan oligomers with different molecular weights
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DOI：
10.1016/s0168-1605(01)00717-6
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发表时间：
2002-03-25
影响因子：
5.4
通讯作者：
Meyers, SP
中科院分区：
文献类型：
--
作者：
No, HK;Park, NY;Meyers, SP
研究了6种不同相对分子质量的壳聚糖和6种壳聚糖低聚物对4种革兰氏阴性菌(大肠杆菌、荧光假单胞菌、鼠伤寒沙门氏菌和副溶血性弧菌)和7种革兰氏阳性菌(单核细胞增生性李斯特菌、巨大芽孢杆菌、蜡样芽孢杆菌、金黄色葡萄球菌、植物乳杆菌、短链杆菌和保加利亚乳杆菌)的抗菌活性。壳聚糖具有比壳聚糖低聚物更高的抗菌活性，对大多数受试细菌的生长有明显的抑制作用，但抑制作用因壳聚糖和特定细菌的相对分子质量而异。在0.1%壳聚糖存在下，壳聚糖对革兰氏阳性菌的杀菌效果普遍强于革兰氏阴性菌。壳聚糖的最小抑菌浓度(MIC)为0.05%~0.1%，具体取决于壳聚糖中的细菌和相对分子质量。作为壳聚糖的溶剂，除乳酸菌以1%的乳酸或甲酸抑制效果较好外，1%的冰醋酸对大多数供试细菌的生长均有抑制作用。PH值在4.5~5.9范围内对壳聚糖的抗菌活性影响较小，pH值越低，壳聚糖的抗菌活性越强。(C)2002 Elsevier Science B.V.保留所有权利。
Antibacterial activities of six chitosans and six chitosan oligomers with different molecular weights (Mws) were examined against four gram-negative (Escherichia coli, Pseudomonas fluorescens, Salmonella typhimurium, and Vibrio parahaemolyticus) and seven gram-positive bacteria (Listeria monocytogenes, Bacillus megaterium, B. cereus, Staphylococcus aureus, Lactobacillus plantarum, L. brevis, and L. bulgaricus). Chitosans showed higher antibacterial activities than chitosan oligomers and markedly inhibited growth of most bacteria tested although inhibitory effects differed with Mws of chitosan and the particular bacterium. Chitosan generally showed stronger bactericidal effects with gram-positive bacteria than gram-negative bacteria in the presence of 0.1% chitosan. The minimum inhibitory concentration (MIC) of chitosans ranged from 0.05% to >0.1% depending on the bacteria and Mws of chitosan. As a chitosan solvent, 1% acetic acid was effective in inhibiting the growth of most of the bacteria tested except for lactic acid bacteria that were more effectively suppressed with 1% lactic or formic acids. Antibacterial activity of chitosan was inversely affected by pH (pH 4.5-5.9 range tested), with higher activity at lower pH value. (C) 2002 Elsevier Science B.V. All rights reserved.