Platycodin D suppressed LPS-induced inflammatory response by activating LXRα in LPS-stimulated primary bovine mammary epithelial cells
Platycodin D suppressed LPS-induced inflammatory response by activating LXRα in LPS-stimulated primary bovine mammary epithelial cells
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DOI：
10.1016/j.ejphar.2017.07.037
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发表时间：
2017-11-05
影响因子：
5
通讯作者：
Fu, Yunhe
中科院分区：
文献类型：
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作者：
Wang, Yanan;Zhang, Xu;Fu, Yunhe
桔梗皂苷D(Platycodin D，PLD)是从桔梗根中提取的一种三萜皂苷，具有抗炎活性。然而，PLD对乳房炎的保护作用尚未见报道。本研究旨在探讨PLD对内毒素刺激的原代牛乳腺上皮细胞(BMEC)的抗炎作用。用四甲基偶氮唑盐比色法测定细胞活力。采用实时定量聚合酶链式反应(qRT-PCR)检测促炎症细胞因子的基因表达，Western blotting检测LXRα和核因子-kappaB的表达。结果显示，PLD可抑制脂多糖刺激的bEMC中IL-1β、IL-6和IL-6的表达。同时，PLD可抑制内毒素诱导的核因子-kappaB的激活。此外，PLD还能上调LXRa的表达。LXRα的抑制剂GGPP可逆转PLD对NF-kappaB活化和炎性细胞因子产生的抑制。综上所述，我们的结果提示PLD通过激活LXRα抑制内毒素诱导的BMEC炎症反应。PLD可能是一种治疗乳房炎的潜在药物。
Platycodin D (PLD), a triterpenoid saponin derived from the root of Platycodon grandiflorum, has been reported to possess anti-inflammatory activity. However, the protective effect of PLD on mastitis has not been reported. In the present study, we aim to investigate the anti-inflammatory feature of PLD on the primary bovine mammary epithelial cells (bMEC) challenged with LPS. The cell viability of bMEC was measured by MTT assay. The quantitative real-time polymerase chain reaction (qRT-PCR) was conducted to detect the gene expression of pro-inflammatory cytokines and western blotting was carried out to measure the expression of LXR alpha and NF-kappa B. The results showed that PLD inhibited LPS-induced TNF-alpha, IL-1 beta, and IL-6 expression in LPS-stimulated bEMC. Meanwhile, PLD suppressed LPS-induced NF-kappa B activation. Furthermore, PLD was found to up-regulate the expression of LXRa. The inhibition of PLD on NF-kappa B activation and inflammatory cytokines production were reversed by GGPP, the inhibitor of LXR alpha. In conclusion, our results suggested that PLD inhibited LPS-induced inflammatory response in bMEC by activating LXR alpha. PLD may be a potential therapeutic drug for mastitis.