Rapid biosynthesis of N-linolenoyl-L-glutamine, an elicitor of plant volatiles, by membrane-associated enzyme(s) in Manduca sexta
Rapid biosynthesis of N-linolenoyl-L-glutamine, an elicitor of plant volatiles, by membrane-associated enzyme(s) in Manduca sexta
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DOI：
10.1073/pnas.1232474100
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发表时间：
2003-06-10
影响因子：
11.1
通讯作者：
Tumlinson, JH
中科院分区：
文献类型：
--
作者：
Lait, CG;Alborn, HT;Tumlinson, JH
为了响应毛虫口腔分泌物中的诱导剂，植物产生并释放挥发性化学物质，在毛虫进食时吸引捕食者和拟寄生虫。最普遍的激发子是由18-碳多不饱和脂肪酸与L-谷氨酰胺偶联组成的脂肪酸酰胺。我们证明快速CoA和ATP独立的体外生物合成的脂肪酸酰胺激发子，N-亚麻酰-L-谷氨酰胺，由几个消化组织的微粒体组分在天蛾。N-亚麻酰-L-谷氨酰胺是几种其他激发子的结构类似物，包括volicitin，第一个在毛虫中鉴定的脂肪酸酰胺激发子。用Triton X-114去污剂相分离法从微粒体中提取的N-亚麻酰-L-谷氨酰胺生物合成酶位于膜蛋白组分中，在碱性pH下具有最大活性。六。利用M. sexta代表了毛虫组织合成脂肪酸酰胺的直接证据。
In response to elicitors in the oral secretions of caterpillars, plants produce and release volatile chemicals that attract predators and parasitoids of the caterpillar while it feeds. The most prevalent elicitors are fatty acid amides consisting of 18-carbon polyunsaturated fatty acids coupled with L-glutamine. We demonstrate rapid CoA- and ATP-independent in vitro biosynthesis of the fatty acid amide elicitor, N-linolenoyl-L-glutamine, by microsomal fractions of several alimentary tissues in Manduca sexta. N-linolenoyl-L-glutamine is a structural analog of several other elicitors including volicitin, the first fatty acid amide elicitor identified in caterpillars. The enzyme(s) that catalyzed biosynthesis of N-linolenoyl-L-glutamine was localized within the integral membrane protein fraction extracted from microsomes by Triton X-114 detergent phase partitioning and had maximum activity at alkaline pH. We found no evidence suggesting microbial or tissue-independent biosynthesis of N-linolenoyl-L-glutamine in M. sexta. The in vitro biosynthesis of N-linolenoyl-L-glutamine by membrane-associated enzyme(s) in M. sexta represents direct evidence of fatty acid amide synthesis by caterpillar tissues.