Genomic signatures of extensive inbreeding in Isle Royale wolves, a population on the threshold of extinction.
Genomic signatures of extensive inbreeding in Isle Royale wolves, a population on the threshold of extinction.
复制标题
皇家岛狼的广泛近亲繁殖的基因组特征，这个种群正处于灭绝的边缘。
DOI：
10.1126/sciadv.aau0757
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发表时间：
2019
期刊：
影响因子：
13.6
通讯作者：
Wayne,RobertK
中科院分区：
文献类型：
--
作者：
Robinson,JacquelineA;Räikkönen,Jannikke;Vucetich,LeahM;Vucetich,JohnA;Peterson,RolfO;Lohmueller,KirkE;Wayne,RobertK
小的孤立的种群往往遭受减少的适应性近亲繁殖衰退的观察指导保护理论和实践了几十年。然而，调查全基因组动态与近交衰退的自然种群是现在才可行的相对便宜的测序技术和注释的参考基因组。为了表征强烈的近亲繁殖和隔离的全基因组效应，我们对一个标志性的近亲繁殖种群，皇家岛的灰狼（Canis lupus）进行了全基因组测序和形态学分析。通过人口遗传模拟和比较狼基因组从各种人口统计学的历史，我们发现的证据表明，严重的近亲繁殖抑郁症在这个人口是由于增加的纯合性的强烈有害的隐性突变。我们的研究结果具有特别的相关性，鉴于最近易位的狼从大陆到皇家岛，以及更广泛的影响，在现代世界的碎片化景观的遗传变异的管理。
The observation that small isolated populations often suffer reduced fitness from inbreeding depression has guided conservation theory and practice for decades. However, investigating the genome-wide dynamics associated with inbreeding depression in natural populations is only now feasible with relatively inexpensive sequencing technology and annotated reference genomes. To characterize the genome-wide effects of intense inbreeding and isolation, we performed whole-genome sequencing and morphological analysis of an iconic inbred population, the gray wolves (Canis lupus) of Isle Royale. Through population genetic simulations and comparison with wolf genomes from a variety of demographic histories, we find evidence that severe inbreeding depression in this population is due to increased homozygosity of strongly deleterious recessive mutations. Our results have particular relevance in light of the recent translocation of wolves from the mainland to Isle Royale, as well as broader implications for management of genetic variation in the fragmented landscape of the modern world.