LncRNA NEAT1 Impacts Cell Proliferation and Apoptosis of Colorectal Cancer via Regulation of Akt Signaling
LncRNA NEAT1 Impacts Cell Proliferation and Apoptosis of Colorectal Cancer via Regulation of Akt Signaling
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LncRNA NEAT1 通过调节 Akt 信号转导影响结直肠癌的细胞增殖和凋亡
DOI：
10.1007/s12253-016-0172-4
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发表时间：
2017-07-01
影响因子：
2.8
通讯作者：
Fan, Hong
中科院分区：
文献类型：
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作者：
Peng, Wei;Wang, Zhuo;Fan, Hong
长链非编码RNA（longnoncodingRNA，lncRNA）是近年来发现的一种调节肿瘤发生的基因，可作为肿瘤治疗的靶点。lncRNA NEAT 1（nuclear paraspeckle assembly transcript 1，Gene ID：283131）在结直肠癌（CRC）中的作用尚不清楚。本研究旨在探讨lncRNA NEAT 1（NEAT 1）在大肠癌中的表达模式及其功能价值和生物学意义。分析了结直肠癌病例中56种癌组织和细胞系中NEAT 1的表达。结果表明，NEAT 1在结直肠癌细胞和组织中显著过表达。临床病理检测证实NEAT 1高表达与结直肠癌体积相关。血清NEAT 1含量明显高于健康对照组（P< 0.05）。NEAT 1在大肠癌与正常大肠组织中的表达显著升高（ROCAUC= 0.9471;P< 0.01）。Kaplan-Meier分析发现NEAT 1升高会导致不良生存（P< 0.05）。进一步的实验表明，NEAT 1敲低信号抑制生长和促进凋亡。重要的是，我们证实了Akt信号通路在CRC中NEAT 1丢失后失活。总之，这项工作支持了NEAT 1可用作人类CRC新治疗的有前景的生物标志物和靶点的第一个证据。
Long noncoding RNA (lncRNA) have been reported to modulate oncogenesis and be used to be target for tumor. The role of lncRNA NEAT1 (nuclear paraspeckle assembly transcript 1, Gene ID: 283131) in colorectal cancer (CRC) keeps unknown. This work was to investigate the pattern of lncRNA NEAT1 (NEAT1) expression in CRC and its functional value and biological significance. NEAT1 expression was analyzed in 56 cancer tissues and cell lines in CRC cases. Results showed that NEAT1 was significantly overexpressed in CRC cells and tissues. Clinicpathologic detection verified that high NEAT1 expression associated with bulk in CRC. The serum contents of NEAT1 were observably elevated comparing with healthy cases (P< 0.05). The levels of NEAT1 were elevated in distinguishing CRC from normal (ROCAUC= 0.9471;P< 0.01). Moreover, Kaplan–Meier analysis found that NEAT1 elevation led to adverse survival (P< 0.05). Further experiments illustrated that of NEAT1 knockdown signally inhibited growth and facilitated apoptosis. Importantly, we confirmed that Akt signaling pathway was inactivated after loss of NEAT1 in CRC. Taken together, this work support the first evidence that NEAT1 can be used to be a promising biomarker and target for novel treatment for human CRC.