Infectious Burden and Semen Parameters
Infectious Burden and Semen Parameters
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DOI：
10.1016/j.urology.2016.10.032
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发表时间：
2017-02-01
期刊：
影响因子：
2.1
通讯作者：
Collodel, Giulia
中科院分区：
文献类型：
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作者：
Moretti, Elena;Figura, Natale;Collodel, Giulia
目的探讨血清慢性感染与精液质量的关系。病原体负担是这样一个概念，即在心脏病患者中，冠状动脉的破坏性影响随着导致慢性感染的病原体数量的增加而增加，患者对这些病原体产生了血清学反应。先前观察到幽门螺杆菌感染可能会降低精液质量，这促使我们进行本研究。方法选取2014年1月至2015年1月入选的73名男性进行血液和精液采集。用光镜和透射电镜观察精液特征。透射电镜数据用数学公式量化，提供数值分数，如生育指数（FI，无超微结构缺陷的精子数量）和精子凋亡、不成熟和坏死的百分比。采用酶联免疫吸附法检测血清样本中免疫球蛋白G对最常见的慢性感染病原的存在，如幽门螺杆菌（HP）、肺炎支原体（MP）、肺炎嗜衣原体（CP）、eb病毒（EBV）、单纯疱疹病毒1 （HSV-1）和巨细胞病毒（CMV）。结果HP 43.8%, CP 46.6%, MP 72.6%, EBV 95.9%, HSV-1 74.0%, CMV 46.6%。随着病原体数量的增加，患者产生了显著的抗体反应，精子浓度(P
OBJECTIVE To investigate the relationship between chronic infections detected in serum and semen quality. The pathogen burden is a concept consisting in the observation that, in patients with heart disease, damaging effects of the coronary arteries increase concomitantly with the number of agents responsible for chronic infections to which patients mounted a serological response. Previous observations that Helicobacter pylori infection may reduce the semen quality prompted us to perform the present study.METHODS Blood and semen samples were collected from 73 selected men, enrolled from January 2014 to January 2015. Semen characteristics were evaluated by light and transmission electron microscopy. Transmission electron microscopy data were quantified with a mathematical formula providing numerical scores, such as fertility index (FI, number of sperm free from ultrastructural defects) and the percentages of sperm apoptosis, immaturity, and necrosis. Serum samples were examined by enzyme-linked immunosorbent assay for the presence of immunoglobulin G to the most common agents of chronic infections such as H. pylori (HP), Mycoplasma pneumoniae (MP), Chlamydophila pneumoniae (CP), Epstein-Barr virus (EBV), herpes simplex virus 1 (HSV-1), and cytomegalovirus (CMV).RESULTS The prevalence of infections was as follows: HP 43.8%, CP 46.6%, MP 72.6%, EBV 95.9%, HSV-1 74.0%, and CMV 46.6%. Concomitantly with the increased number of pathogens against which the patients mounted a significant antibody response, sperm concentration (P