Deep imaging revealed dynamics and signaling in one-to-one pollen tube guidance
Deep imaging revealed dynamics and signaling in one-to-one pollen tube guidance
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DOI：
10.1101/2023.04.19.537439
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发表时间：
2023-04
期刊：
影响因子：
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通讯作者：
Y. Mizuta;Daigo Sakakibara;Shiori Nagahara;Ikuma Kaneshiro;Takuya T. Nagae;Daisuke Kurihara;T. Higashiyama
中科院分区：
文献类型：
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作者：
Y. Mizuta;Daigo Sakakibara;Shiori Nagahara;Ikuma Kaneshiro;Takuya T. Nagae;Daisuke Kurihara;T. Higashiyama
在被子植物中，花粉管引导允许精子细胞输送到胚珠内的雌配子，这些雌配子深深地嵌入花朵中。然而，当子房包含多个花粉管和胚珠时，尚不清楚每个胚珠如何通过花粉管一对一地受精。在这里，我们的双光子成像揭示了活体卵巢中的花粉管动力学。花粉管数量和胚珠成熟度影响多个胚珠中的目标选择。在传播道内隔膜表皮的内表面上，花粉管的行为和出现受到胚珠孢子体信号的调节。在缆索引导下，第二个花粉管被 FERONIA 和 LORELEI 依赖性配子体信号严格排斥，尤其是在第一个花粉管通过后 45 分钟以上。这种一对一花粉管引导的高度时空调节机制可能允许被子植物在自然界中产生更多的后代。
In angiosperms, pollen tube guidance allows sperm cell delivery to the female gametes within the ovule, which are deeply embedded in a flower. However, when an ovary includes multiple pollen tubes and ovules, it is unclear how each ovule is fertilized one-to-one by a pollen tube. Here, our two-photon imaging revealed the pollen tube dynamics in living ovaries. The number of pollen tubes and ovule maturity affected the target selection among multiple ovules. On the inner surface of the septum epidermis within the transmitting tract, pollen tube behavior and emergence were regulated by the ovular sporophytic signals. In funicular guidance, the second pollen tube was strictly repelled by the FERONIA and LORELEI-dependent gametophytic signal, especially more than 45 minutes after the first pollen tube had passed. Such highly spatiotemporal regulation mechanisms in the one-to-one pollen tube guidance may allow angiosperms to produce more offspring in nature.